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          • Primary Antibodies ›
          • APOE Antibodies

          Invitrogen

          APOE Polyclonal Antibody

          Advanced Verification
          View all (50) APOE antibodies

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          Cite APOE Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (7)
          • Advanced Verification (2)
          APOE Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          APOE Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          APOE Antibody (PA5-95232) in ICC/IF

          Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 and A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with APOE Polyclonal Antibody (Product # PA5-95232) at 5 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          APOE Antibody in Immunocytochemistry (ICC/IF)
          APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          APOE Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          APOE Antibody in Western Blot (WB)
          APOE Antibody in Western Blot (WB)
          APOE Antibody in Flow Cytometry (Flow)
          APOE Antibody in ELISA (ELISA)
          APOE Antibody
          APOE Antibody
          APOE Polyclonal Antibody

          Product Details

          PA5-95232

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          0.1-0.5 µg/mL
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          0.5-1 µg/mL
          -

          Immunocytochemistry (ICC/IF)

          5 µg/mL
          -

          Flow Cytometry (Flow)

          1-3 µg/1x10^6 cells
          -

          ELISA (ELISA)

          1-5 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          E.coli-derived human Apolipoprotein E recombinant protein (Position: K19-H317).
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Lyophilized

          Concentration

          500 µg/mL

          Amount

          100 µg

          Purification

          Affinity chromatography

          Storage buffer

          PBS with 5mg BSA

          Contains

          0.05mg sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2807036

          Product Specific Information

          Human Apolipoprotein E shares 73% and 72% amino acid (aa) sequence identity with mouse and rat Apolipoprotein E, respectively.

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: human placenta tissue, human HepG2 whole cell. IHC: Human Placenta tissue. Flow: HepG2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

          Target Information

          Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Apo-E; ApoE4; Apolipoprotein; Apolipoprotein E; apolipoprotein E3; apolipoprotein E4; Apoprotein; B2G1; BG; MGC1571; unnamed protein product

          View more View less

          Gene Aliases: AD2; APO-E; APOE; ApoE4; LDLCQ5; LPG

          View more View less

          UniProt ID: (Human) P02649

          View more View less

          Entrez Gene ID: (Human) 348

          View more View less

          Function(s)
          beta-amyloid binding receptor binding structural molecule activity lipid transporter activity protein binding phospholipid binding heparin binding lipid binding antioxidant activity enzyme binding identical protein binding protein homodimerization activity heparan sulfate proteoglycan binding macromolecular complex binding metal chelating activity protein dimerization activity receptor agonist activity tau protein binding low-density lipoprotein particle receptor binding phosphatidylcholine-sterol O-acyltransferase activator activity very-low-density lipoprotein particle receptor binding lipoprotein particle binding intermembrane cholesterol transfer activity
          Process(es)
          response to reactive oxygen species negative regulation of endothelial cell proliferation response to dietary excess lipid metabolic process triglyceride metabolic process cholesterol catabolic process lipid transport cellular calcium ion homeostasis receptor-mediated endocytosis response to oxidative stress cytoskeleton organization G-protein coupled receptor signaling pathway synaptic transmission, cholinergic long-term memory steroid metabolic process cholesterol metabolic process gene expression regulation of gene expression negative regulation of platelet activation negative regulation of endothelial cell migration negative regulation of gene expression negative regulation of platelet-derived growth factor receptor signaling pathway positive regulation of cholesterol efflux lipid transport involved in lipid storage positive regulation of neuron projection development negative regulation of neuron projection development long-chain fatty acid transport protein import virion assembly negative regulation of blood coagulation regulation of axon extension neuron projection development melanosome organization regulation of Cdc42 protein signal transduction positive regulation of low-density lipoprotein particle receptor catabolic process cholesterol efflux phospholipid efflux very-low-density lipoprotein particle remodeling low-density lipoprotein particle remodeling high-density lipoprotein particle remodeling high-density lipoprotein particle assembly chylomicron remnant clearance high-density lipoprotein particle clearance very-low-density lipoprotein particle clearance locomotory exploration behavior nitric oxide-cGMP-mediated signaling pathway lipoprotein metabolic process lipoprotein biosynthetic process lipoprotein catabolic process vasodilation cholesterol homeostasis regulation of apoptotic process amyloid precursor protein metabolic process regulation of protein complex assembly negative regulation of MAPK cascade negative regulation of blood vessel endothelial cell migration reverse cholesterol transport positive regulation by host of viral process regulation of innate immune response positive regulation of nitric oxide biosynthetic process negative regulation of cholesterol biosynthetic process positive regulation of endocytosis positive regulation of transcription, DNA-templated positive regulation of lipid biosynthetic process intracellular transport regulation of neuronal synaptic plasticity negative regulation of smooth muscle cell proliferation artery morphogenesis negative regulation of protein secretion negative regulation of inflammatory response regulation of synapse organization positive regulation of membrane protein ectodomain proteolysis negative regulation of lipid biosynthetic process regulation of protein metabolic process negative regulation of protein metabolic process maintenance of location in cell lipid homeostasis fatty acid homeostasis acylglycerol homeostasis artery development positive regulation of dendritic spine development negative regulation of dendritic spine development regulation of proteasomal protein catabolic process response to caloric restriction triglyceride homeostasis positive regulation of ERK1 and ERK2 cascade cellular response to lipoprotein particle stimulus triglyceride-rich lipoprotein particle clearance intermediate-density lipoprotein particle clearance circulatory system development negative regulation of canonical Wnt signaling pathway regulation of cholesterol metabolic process positive regulation of cholesterol metabolic process negative regulation of triglyceride metabolic process negative regulation of cholesterol efflux regulation of plasma lipoprotein particle levels AMPA glutamate receptor clustering NMDA glutamate receptor clustering cellular oxidant detoxification positive regulation of lipoprotein transport regulation of beta-amyloid clearance positive regulation of beta-amyloid clearance negative regulation of long-term synaptic potentiation negative regulation of postsynaptic membrane organization positive regulation of presynaptic membrane organization positive regulation of beta-amyloid formation negative regulation of beta-amyloid formation negative regulation of dendritic spine maintenance positive regulation of dendritic spine maintenance regulation of amyloid precursor protein catabolic process positive regulation of phospholipid efflux positive regulation of neurofibrillary tangle assembly negative regulation of phospholipid efflux negative regulation of lipid transport across blood brain barrier positive regulation of lipid transport across blood brain barrier cellular response to beta-amyloid regulation of cellular response to very-low-density lipoprotein particle stimulus regulation of amyloid fibril formation negative regulation of amyloid fibril formation positive regulation of amyloid fibril formation regulation of behavioral fear response
          It has to be done as per old AB suggested Products section.

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