|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A peptide corresponding to aa 737 to 751 of human amyloid A4 protein precursor (APP) or 85 to 99 of the C99 fragment generated by b-secretase cleavage|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||20 ug/ml|
|Immunofluorescence (IF)||20 ug/ml|
|Immunohistochemistry (IHC)||2 ug/ml|
|Western Blot (WB)||2 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 4 publications below|
A suggested positive control is mouse brain tissue lysate.
PA5-19923 can be used with blocking peptide PEP-0048.
Accumulation of the amyloid-beta peptide (Abeta) in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. The beta-amyloid protein precursor (APP) is cleaved by beta-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for -gamma-secretase to generate the 4 kDa amyloid-beta peptide (Abeta), which is deposited in the brains of all suffers of Alzheimer and quote;s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Ethanol extract of Magnolia officinalis prevents lipopolysaccharide-induced memory deficiency via its antineuroinflammatory and antiamyloidogenic effects.
PA5-19923 was used in western blot to study the roles of inflammatory and amyloidogenic pathways in the mechanism by which Magnolia officinalis extracts protect against LPS-induced memory impairment
|Lee YJ,Choi DY,Yun YP,Han SB,Kim HM,Lee K,Choi SH,Yang MP,Jeon HS,Jeong JH,Oh KW,Hong JT||Phytotherapy research : PTR (27:438)||2013|
Epigallocatechin-3-gallate prevents systemic inflammation-induced memory deficiency and amyloidogenesis via its anti-neuroinflammatory properties.
PA5-19923 was used in western blot to study the role of the anti-neuroinflammatory properties of epigallocatechin-3-gallate in the mechanism by which it protects against LPS-induced amyloidogenesis and memory deficit
|Lee YJ,Choi DY,Yun YP,Han SB,Oh KW,Hong JT||The Journal of nutritional biochemistry (24:298)||2013|
4-O-methylhonokiol prevents memory impairment in the Tg2576 transgenic mice model of Alzheimer's disease via regulation of β-secretase activity.
PA5-19923 was used in western blot to study the role of reduced beta-secretase activity in the mechanism by which 4-O-methylhonokiol protects against memory loss in a murine model of Alzheimer's disease
|Lee YJ,Choi DY,Lee YK,Lee YM,Han SB,Kim YH,Kim KH,Nam SY,Lee BJ,Kang JK,Yun YW,Oh KW,Hong JT||Journal of Alzheimer's disease : JAD (29:677)||2012|
Inhibitory effect of 4-O-methylhonokiol on lipopolysaccharide-induced neuroinflammation, amyloidogenesis and memory impairment via inhibition of nuclear factor-kappaB in vitro and in vivo models.
PA5-19923 was used in western blot to study the role of NFkB in the mechanism by which 4-O-methylhonokiol protects against LPS-induced amyloid formation, neuroinflammation and memory deficits in a murine Alzheimer's disease model
|Lee YJ,Choi DY,Choi IS,Kim KH,Kim YH,Kim HM,Lee K,Cho WG,Jung JK,Han SB,Han JY,Nam SY,Yun YW,Jeong JH,Oh KW,Hong JT||Journal of neuroinflammation (9:null)||2012|