Invitrogen

APRT Polyclonal Antibody

View all (9) APRT antibodies

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APRT Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of APRT in paraffin-embedded human prostate using APRT Polyclonal Antibody (Product # PA5-89545) at a dilution of 1:100.

APRT Antibody in Western Blot (WB) — Western Blot analysis of APRT in extracts of various cell lines using APRT Polyclonal Antibody (Product # PA5-89545) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-89545

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human APRT View immunogen
Conjugate	Unconjugated
Form	liquid
Concentration	0.65 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2805641

Product Specific Information

Immunogen sequence: MADSELQLVE QRIRSFPDFP TPGVVFRDIS PVLKDPASFR AAIGLLARHL KATHGGRIDY IAGLDSRGFL FGPSLAQELG LGCVLIRKRG KLPGPTLWAS YSLEYGKAEL EIQKDALEPG QRVVVVDDLL ATGGTMNAAC ELLGRLQAEV LECVSLVELT SLKGREKLAP VPFFSLLQYE; Positive Samples: MCF-7, A-549, K-562, HeLa, Mouse small intestine; Cellular Location: Cytoplasm

Target Information

Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; APRT; DKFZp686D13177; MGC125856; MGC125857; MGC129961; transphosphoribosidase

Gene Aliases: AMP; APRT; APRTD; C85684

UniProt ID: (Human) Q68DF9, (Mouse) P08030, (Rat) P36972

Entrez Gene ID: (Human) 353, (Mouse) 11821, (Rat) 292072

Molecular Function: transferase metabolite interconversion enzyme