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Invitrogen
APRT Polyclonal Antibody
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FIGURE: 1 / 7
APRT Antibody (PA5-95613) in IHC (P)
Immunohistochemistry analysis of APRT in paraffin-embedded human intestinal cancer tissue. Antigen retrieval was performed on the tissue using citrate buffer (pH 6, 20 min) and blocked with 10% goat serum. Samples were incubated with APRT polyclonal antibody (Product # PA5-95613) at a 1 µg/mL dilution, followed by biotinylated goat anti-rabbit IgG (30 min, 37°C), and developed with Strepavidin-Biotin-Comple... View More
Product Details
PA5-95613
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of mouse APRT (RAEVVECVSLVELTSLKGRERL).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human MCF-7 whole cell, human 293T whole cell, human SiHa whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue. IHC: human intestinal cancer tissue, human intestinal cancer tissue, human intestinal cancer tissue, human mammary cancer tissue, human mammary cancer tissue.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Adenine phosphoribosyltransferase belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Adenine phosphoribosyltransferase; AMP diphosphorylase; AMP pyrophosphorylase; APRT; DKFZp686D13177; MGC125856; MGC125857; MGC129961; transphosphoribosidase
Gene Aliases: AMP; APRT; APRTD; C85684
UniProt ID: (Human) P07741, (Mouse) P08030, (Rat) P36972
Entrez Gene ID: (Human) 353, (Mouse) 11821, (Rat) 292072
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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