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Invitrogen
APS Polyclonal Antibody
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FIGURE: 1 / 1
APS Antibody (PA1-1062) in ICC/IF
Immunofluorescence analysis of APS was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with APS Rabbit Polyclonal Antibody (Product # PA1-1062) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Prod... View More
Product Details
PA1-1062
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to residues E(605) A T L G R A R A V E N Q Y S(619) from mouse APS.
Conjugate
Form
Liquid
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-1062 detects APS from mouse samples.
PA1-1062 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~ 84 kDa protein corresponding to APS from recombinant mouse protein and 3T3L1 extracts.
The PA1-1062 immunogen is a synthetic peptide corresponding to residues E(605)A T L G R A R A V E N Q Y S(619) from mouse APS. This peptide (Cat. # PEP-246) can be used in neutralization and control experiments.
Target Information
APS (adapter protein with Pleckstrin homology and Src homology 2 domains) a member of the Lnk family, is an adaptor protein that is involved in B-cell signaling, insulin signaling, cytoskeletal reorganization. It is tyrosine phosphorylated by JAK2, KIT and other kinases during B-cell receptor stimulation of many different cytokines, chemokines and leukokines. APS has been shown to inhibit the JAK-STAT pathway in collaboration with c-Cbl. It is located in the cytoplasm in pre-PDGF (platelet derived growth factor) stimulated cells and localizes to the plasma membrane and peripheral regions upon PDGF stimulation. This protein is expressed in B-cells and mast cells when detected in haematopoietic cell lines yet is also expressed in many different tissues including spleen, prostrate, testis, uterus, small intestine and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adapter protein with pleckstrin homology and Src homology 2 domains; Alpha-1-antitrypsin; SH2 and PH domain-containing adapter protein APS; SH2B adapter protein 2
Gene Aliases: Aps; Sh2b2
UniProt ID: (Mouse) Q9JID9
Entrez Gene ID: (Mouse) 23921
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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