Invitrogen

ARC Polyclonal Antibody

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Protocols
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Product Details

PA5-29861

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human NOL3. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.3 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2547335

Product Specific Information

Recommended positive controls: HeLa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ARC (also known as NOL3 - Nucleolar protein 3) has two isoforms. Isoform 1 is involved in RNA splicing. Isoform 2 functions as an apoptosis repressor that blocks multiple modes of cell death. ARC inhibits extrinsic apoptotic pathways two different ways. The first is by interacting with FAS and FADD upon FAS activation blocking death-inducing signaling complex (DISC) assembly. The second way is by interacting with CASP8 in a mitochondria localization-and phosphorylation-dependent manner, which in turn limits the amount of soluble CASP8 avaliable for DISC-mediated activation. ARC also inhibits intrinsic apoptotic pathways in response to a wide range of stresses through interactions with BAX. This results in BAX inactivation and prevents mitochondrial dysfunction and release of pro-apoptotic factors. Mutations affecting the gene can results in myoclonus, familiam cortical.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Apoptosis repressor with CARD; Muscle-enriched cytoplasmic protein; Myp; Nop 30; Nop30; Nucleolar protein 3; nucleolar protein 3 (apoptosis repressor with CARD domain); Nucleolar protein of 30 kDa

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Gene Aliases: ARC; B430311C09Rik; FCM; MYC; MYP; NOL3; NOP; NOP30

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UniProt ID: (Human) O60937, (Rat) Q62881, (Mouse) Q9D1X0

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Entrez Gene ID: (Human) 8996, (Rat) 85383, (Mouse) 78688

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Function(s)
RNA binding death receptor binding calcium ion binding protein binding death effector domain binding identical protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process caspase binding receptor binding kinase binding phosphatase binding metal ion binding
Process(es)
response to hypoxia blood vessel remodeling response to ischemia mRNA splice site selection mRNA processing RNA splicing regulation of gene expression cardiac muscle cell apoptotic process negative regulation of striated muscle cell apoptotic process negative regulation of cardiac muscle cell apoptotic process regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum negative regulation of muscle atrophy release of sequestered calcium ion into cytosol by sarcoplasmic reticulum response to injury involved in regulation of muscle adaptation regulation of apoptotic process negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process myoblast differentiation smooth muscle cell proliferation protein oligomerization negative regulation of cytosolic calcium ion concentration negative regulation of mitochondrial calcium ion concentration cellular response to hypoxia negative regulation of release of cytochrome c from mitochondria intrinsic apoptotic signaling pathway inhibition of cysteine-type endopeptidase activity negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of mitochondrial membrane permeability involved in apoptotic process negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of death-inducing signaling complex assembly negative regulation of hydrogen peroxide-induced cell death negative regulation of protein targeting to mitochondrion negative regulation of hypoxia-induced intrinsic apoptotic signaling pathway inhibition of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of extrinsic apoptotic signaling pathway negative regulation of intrinsic apoptotic signaling pathway negative regulation of tumor necrosis factor-mediated signaling pathway regulation of NF-kappaB import into nucleus negative regulation of necrotic cell death
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