Western blot of ARF4 in (A) NIH-3T3 and (B) HeLa lysates was performed using 35ug protein in RIPA buffer. Lysates were probed with an ARF4 polyclonal antibody (Product# PA5-37841) at a dilution of 0.5ug/ml and incubated for 1 hour, followed by chemiluminescent detection.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Peptide with sequence C-SEMTDKLGLQSLRN, from the internal region of ARF4 (aa137-150)|
|Purification||Antigen affinity chromatography|
|Storage buffer||TBS, pH 7.3, with 0.5% BSA|
|Contains||0.02% sodium azide|
|Tested Applications||Dilution *|
|Western Blot (WB)||0.3-1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat, pig and cow based on sequence homology.
ADP-ribosylation factor 1 is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I and class III , and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.
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