|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 161 of Human ARF5|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-31432 targets ARF5 in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-31432 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 161 of Human ARF5.
ADP-ribosylation factor 5 (ARF5) is a member of the human ARF gene family. These genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 6 ARF proteins and 11 ARF-like proteins and constitute 1 family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2,and ARF3), class II (ARF4 and ARF5) and class III (ARF6). The members of each class share a common gene organization. The ARF5 gene spans approximately 3.2kb of genomic DNA and contains six exons and five introns.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Which Way In? The RalF Arf-GEF Orchestrates Rickettsia Host Cell Invasion.
PA5-31432 was used in western blot to study different bacterial guanine nucleotide exchange factor of ADP-ribosylation factors
|Rennoll-Bankert KE,Rahman MS,Gillespie JJ,Guillotte ML,Kaur SJ,Lehman SS,Beier-Sexton M,Azad AF||PLoS pathogens (11:null)||2015|