Immunofluorescent analysis of ARFGAP1 + ARFGAP3 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in -20°C 100% MeOH for 5 min and stained using an ARFGAP1 + ARFGAP3 polyclonal antibody (Product # PA5-28707) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 454 and 516 of Human ARFGAP1/3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-28707 targets ARFGAP1/3 in WB applications and shows reactivity with Human samples.
The PA5-28707 immunogen is synthetic peptide corresponding to a region within amino acids 454 and 516 of Human ARFGAP1/3.
The protein encoded by this gene is a GTPase-activating protein (GAP) that associates with the Golgi apparatus and regulates the early secretory pathway of proteins. The encoded protein promotes hydrolysis of ADP-ribosylation factor 1 (ARF1)-bound GTP, which is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is a prerequisite for the fusion of these vesicles with target compartments. The activity of this protein is sensitive to phospholipids. Multiple transcript variants encoding different isoforms have been found for this gene. This gene was originally known as ARFGAP1, but that is now the name of a related but different gene.
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