Proteintech

ARG1 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ARG1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 16001-1-AP (ARG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ARG1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using 16001-1-AP ( ARG1 Antibody) at dilution of 1:200 (under 40x lens).

ARG1 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16001-1-AP (ARG1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

ARG1 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were stained with 0.2ug ARG1 antibody (16001-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L) with dilution 1:1500.

ARG1 Antibody in Immunoprecipitation (IP) — IP result of anti-ARG1 (IP:16001-1-AP, 4ug; Detection:16001-1-AP 1:700) with mouse liver tissue lysate 5200ug.

Product Details

16001-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 15 publications 15 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:400	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 7 publications 7 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ARG1 Fusion Protein Ag8595 (1-236 aa encoded by BC005321) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSAKSRTIG IIGAPFSKGQ PRGGVEEGPT VLRKAGLLEK LKEQECDVKD YGDLPFADIP NDSPFQIVKN PRSVGKASEQ LAGKVAEVKK NGRISLVLGG DHSLAIGSIS GHARVHPDLG VIWVDAHTDI NTPLTTTSGN LHGQPVSFLL KELKGKIPDV PGFSWVTPCI SAKDIVYIGL RDVDPGEHYI LKTLGIKYFS MTEVTRTVNT AVAITLACFG LAREGNHKPI DYLNPPK (1-236 aa encoded by BC005321)

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Clemastine Alleviates Depressive-Like Behavior Through Reversing the Imbalance of Microglia-Related Pro-inflammatory State in Mouse Hippocampus.

Frontiers in cellular neuroscience

Su WJ,Zhang T,Jiang CL,Wang W

Wed Sep 30 00:00:00 EDT 2020

Aryl Hydrocarbon Receptor Regulates Apoptosis and Inflammation in a Murine Model of Experimental Autoimmune Uveitis.

Frontiers in immunology

Huang Y,He J,Liang H,Hu K,Jiang S,Yang L,Mei S,Zhu X,Yu J,Kijlstra A,Yang P,Hou S

Wed Nov 20 00:00:00 EST 2019

Effective Amelioration of Liver Fibrosis Through Lentiviral Vector Carrying in Murine Model.

Frontiers in immunology

Liu L,Jin M,Tao Q,Yu L,Du J,Wang C,Luo Q,Xing T,Xu Y,Shen J,Chu D

Wed Nov 20 00:00:00 EST 2019

Rapamycin and FTY720 Alleviate Atherosclerosis by Cross Talk of Macrophage Polarization and Autophagy.

BioMed research international

Sun RZ,Fan Y,Liang X,Gong TT,Wang Q,Liu H,Shan ZY,Lei L

Wed Apr 24 00:00:00 EDT 2019

S100A8/A9 induces microglia activation and promotes the apoptosis of oligodendrocyte precursor cells by activating the NF-κB signaling pathway.

Brain research bulletin

Wu M,Xu L,Wang Y,Zhou N,Zhen F,Zhang Y,Qu X,Fan H,Liu S,Chen Y,Yao R

Mon Oct 01 00:00:00 EDT 2018

The Role of Alpha-Lipoic Acid in the Pathomechanism of Acute Ischemic Stroke.

Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

Wang Q,Lv C,Sun Y,Han X,Wang S,Mao Z,Xin Y,Zhang B

Tue Sep 04 00:00:00 EDT 2018

Interleukin-33 Protects Ischemic Brain Injury by Regulating Specific Microglial Activities.

Neuroscience

Luo Q,Fan Y,Lin L,Wei J,Li Z,Li Y,Nakae S,Lin W,Chen Q

Fri Aug 10 00:00:00 EDT 2018

ER-localized protein-Herpud1 is a new mediator of IL-4-induced macrophage polarization and migration.

Experimental cell research

Li Y,Xie Y,Hao J,Liu J,Ning Y,Tang Q,Ma M,Zhou H,Guan S,Zhou Q,Lv X

Sun Jul 15 00:00:00 EDT 2018

l-Arginine induces antioxidant response to prevent oxidative stress via stimulation of glutathione synthesis and activation of Nrf2 pathway.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Liang M,Wang Z,Li H,Cai L,Pan J,He H,Wu Q,Tang Y,Ma J,Yang L

Tue May 01 00:00:00 EDT 2018

Treadmill Exercise Exerts Neuroprotection and Regulates Microglial Polarization and Oxidative Stress in a Streptozotocin-Induced Rat Model of Sporadic Alzheimer's Disease.

Journal of Alzheimer's disease : JAD

Lu Y,Dong Y,Tucker D,Wang R,Ahmed ME,Brann D,Zhang Q

Fri Feb 23 00:00:00 EST 2018

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Experimental neurology

Yang L,Tucker D,Dong Y,Wu C,Lu Y,Li Y,Zhang J,Liu TC,Zhang Q

Mon Jan 01 00:00:00 EST 2018

GRP78 plays an integral role in tumor cell inflammation-related migration induced by M2 macrophages.

Cellular signalling

Zhang L,Li Z,Ding G,La X,Yang P,Li Z

Fri Sep 01 00:00:00 EDT 2017

Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.

Biochimica et biophysica acta. Molecular basis of disease

Bichell TJV,Wegrzynowicz M,Tipps KG,Bradley EM,Uhouse MA,Bryan M,Horning K,Fisher N,Dudek K,Halbesma T,Umashanker P,Stubbs AD,Holt HK,Kwakye GF,Tidball AM,Colbran RJ,Aschner M,Neely MD,Di Pardo A,Maglione V,Osmand A,Bowman AB

Thu Jun 01 00:00:00 EDT 2017

Matrine promotes NT3 expression in CNS cells in experimental autoimmune encephalomyelitis.

Neuroscience letters

Zhang ML,Zhang XJ,Kang J,Zhang HJ,Chen XL,Liu N,Liu SQ,Ma WD,Zhang GX,Zhu L

Wed May 10 00:00:00 EDT 2017

Effects of IL-4 on pulmonary fibrosis and the accumulation and phenotype of macrophage subpopulations following thoracic irradiation.

International journal of radiation biology

Groves AM,Johnston CJ,Misra RS,Williams JP,Finkelstein JN

Thu Dec 01 00:00:00 EST 2016

Autophagic flux regulates microglial phenotype according to the time of oxygen-glucose deprivation/reperfusion.

International immunopharmacology

Xia CY,Zhang S,Chu SF,Wang ZZ,Song XY,Zuo W,Gao Y,Yang PF,Chen NH

Sat Oct 01 00:00:00 EDT 2016

Conditioned Medium from the Stem Cells of Human Exfoliated Deciduous Teeth Ameliorates Experimental Autoimmune Encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Shimojima C,Takeuchi H,Jin S,Parajuli B,Hattori H,Suzumura A,Hibi H,Ueda M,Yamamoto A

Sun May 15 00:00:00 EDT 2016

A(H1N1) vaccination recruits T lymphocytes to the choroid plexus for the promotion of hippocampal neurogenesis and working memory in pregnant mice.

Brain, behavior, and immunity

Qi F,Yang J,Xia Y,Yuan Q,Guo K,Zou J,Yao Z

Tue Mar 01 00:00:00 EST 2016

Neonatal BCG vaccination of mice improves neurogenesis and behavior in early life.

Brain research bulletin

Yang J,Qi F,Gu H,Zou J,Yang Y,Yuan Q,Yao Z

Fri Jan 01 00:00:00 EST 2016

M2 Polarization of Monocytes-Macrophages Is a Hallmark of Indian Post Kala-Azar Dermal Leishmaniasis.

PLoS neglected tropical diseases

Mukhopadhyay D,Mukherjee S,Roy S,Dalton JE,Kundu S,Sarkar A,Das NK,Kaye PM,Chatterjee M

Thu Oct 01 00:00:00 EDT 2015

Pioglitazone suppresses neuronal and muscular degeneration caused by polyglutamine-expanded androgen receptors.

Human molecular genetics

Iida M,Katsuno M,Nakatsuji H,Adachi H,Kondo N,Miyazaki Y,Tohnai G,Ikenaka K,Watanabe H,Yamamoto M,Kishida K,Sobue G

Thu Jan 15 00:00:00 EST 2015

Ethanol supports macrophage recruitment and reinforces invasion and migration of Lewis lung carcinoma.

Alcoholism, clinical and experimental research

Yu K,Yang J,Wang F,Chen L,Lu Y,Luo J,Wang S

Wed Oct 01 00:00:00 EDT 2014

Ethanol supports macrophage recruitment and reinforces invasion and migration of Lewis lung carcinoma.

Alcoholism, clinical and experimental research

Yu K,Yang J,Wang F,Chen L,Lu Y,Luo J,Wang S

Wed Oct 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176

Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846

Molecular Function: hydrolase

Function(s)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process female pregnancy response to wounding response to lipopolysaccharide response to peptide hormone response to steroid hormone

Process(es)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding

ARG1 Polyclonal Antibody

Product Details

16001-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!