Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | View 15 publications 15 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:100-1:400 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | View 7 publications 7 publications |
Flow Cytometry (Flow) |
0.2 µg/1x10^6 cells | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ARG1 Fusion Protein Ag8595 (1-236 aa encoded by BC005321) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.25 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MSAKSRTIG IIGAPFSKGQ PRGGVEEGPT VLRKAGLLEK LKEQECDVKD YGDLPFADIP NDSPFQIVKN PRSVGKASEQ LAGKVAEVKK NGRISLVLGG DHSLAIGSIS GHARVHPDLG VIWVDAHTDI NTPLTTTSGN LHGQPVSFLL KELKGKIPDV PGFSWVTPCI SAKDIVYIGL RDVDPGEHYI LKTLGIKYFS MTEVTRTVNT AVAITLACFG LAREGNHKPI DYLNPPK (1-236 aa encoded by BC005321)
Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: A-I; AI type I arginase; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase
Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF
UniProt ID: (Human) P05089, (Rat) P07824, (Mouse) Q61176
Entrez Gene ID: (Human) 383, (Rat) 29221, (Mouse) 11846
Molecular Function:
hydrolase
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