|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 193-219 amino acids from the central region of human ARHGAP25|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
ARHGAP25 encode negative regulators of Rho GTPases (see ARHA; MIM 165390), which are implicated in actin remodeling, cell polarity, and cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Phosphoinositide 3-kinase enables phagocytosis of large particles by terminating actin assembly through Rac/Cdc42 GTPase-activating proteins.
PA5-24791 was used in western blot to test if Rac and Cdc42 contribute to phagocytosis
|Schlam D,Bagshaw RD,Freeman SA,Collins RF,Pawson T,Fairn GD,Grinstein S||Nature communications (6:null)||2015|