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Product Details
PA5-52776
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
0.04-0.4 µg/mL | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein corresponding to Human ARHGEF11 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.1 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.2, with 40% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2638161 |
Product Specific Information
Immunogen sequence: LQAEIDSRLR NSEDARGVLC EAQEAAMPEI QEQIHDYRTK RTLGLGSLYG ENDLLDLDGD PLRERQVAEK QLAALGDILS KYEEDRSAPM DFALNTYMSH AGIRLREARP SNTAEKAQSA PDKDKWLPFF PKTKKSSNSK KEQDA
Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 88%.
Target Information
ARHGEF11 is a 1527 amino acid protein with tandem dbl homology and pleckstrin homology domains, PDZ domain, two proline rich sequences and a regulatory G protein-signaling (RGS) sequence. It acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase, but not for RAC1 or CDC42 and acts as GTPase-activating protein (GAP) for GNA12 and GNA13. It forms a complex with G proteins and stimulates neurite through retraction Rho-dependent signals. ARHGEF11 increases glutamate transport 4-fold. It interacts with EAAT4 and modulates its uptake activity and perisynaptic distribution at glutamatergic synapses. It is known to induce the reorganization of the actin cytoskeleton and its overexpression induces the formation of membrane ruffling and filopodia. It is ubiquitously expressed, in brain, lung, liver, kidney and skeletal muscle.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: glutamate transporter EAAT4-associated protein 48; PDZ-RhoGEF; Rho guanine exchange factor (GEF) 11; Rho guanine nucleotide exchange factor (GEF) 11; Rho guanine nucleotide exchange factor 11; RhoA-specific guanine nucleotide exchange factor; RhoGEF glutamate transport modulator; RP11-356J7.2
Gene Aliases: ARHGEF11; GTRAP48; KIAA0380; PDZ-RHOGEF
UniProt ID: (Human) O15085
Entrez Gene ID: (Human) 9826
Molecular Function:
guanyl-nucleotide exchange factor
cytokinesis
regulation of cell growth
movement of cell or subcellular component
striated muscle contraction
G-protein coupled receptor signaling pathway
Rho protein signal transduction
establishment of cell polarity
actin cytoskeleton organization
regulation of Rho protein signal transduction
positive regulation of apoptotic process
positive regulation of GTPase activity
positive regulation of transcription, DNA-templated
regulation of small GTPase mediated signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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