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Product Details
PA5-106866
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ARHGEF7(Accession Q14155), corresponding to amino acid residues N311-Y361.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2854530
Product Specific Information
Antibody detects endogenous levels of total ARHGEF7.
Target Information
The serine/threonine kinase, p21 activated kinase (PAK), is a downstream effector of the small GTPases Cdc42 and Rac. PAK associates with Nck, the p85 and p110 subunits of PI3-kinase and PIX (PAK-interacting exchange factor) in a focal complex. The binding of PIX is necessary for the localization and activation of PAK in the Cdc42 to Rac signaling pathway, and this binding occurs through the high affinity of the N-terminal SH3 domain of PIX for a conserved proline rich PAK sequence. PIX exists as two isoforms, alpha and beta, and both are highly expressed in heart, muscle and thymus tissues of human and rat. alphaPIX is phosphorylated via PDGF and EphB2 receptor signaling pathways or through association with PI3-kinase. The alphaPIX isoform predominantly acts as a guanine nucleotide exchange factor (GEF) on Rac, which may mediate lamellipodia formation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Beta-Pix; COOL-1; p85; p85SPR; PAK-interacting exchange factor beta; Pak3 binding protein; PIXbeta; Rho guanine nucleotide exchange factor (GEF) 7; Rho guanine nucleotide exchange factor 7; SH3 domain-containing proline-rich protein
Gene Aliases: ARHGEF7; BETA-PIX; betaPix; betaPix-b; betaPix-c; Cool; COOL-1; COOL1; KIAA0142; mKIAA0142; Nbla10314; P50; P50BP; P85; P85COOL1; P85SPR; PAK3; PAK3BP; PIX; PIXB
UniProt ID: (Human) Q14155, (Rat) O55043, (Mouse) Q9ES27
Entrez Gene ID: (Human) 8874, (Rat) 114559, (Mouse) 54126
hematopoietic progenitor cell differentiation
Golgi organization
signal transduction
nervous system development
positive regulation of fibroblast migration
lamellipodium assembly
positive regulation of protein binding
regulation of Rho protein signal transduction
intracellular signal transduction
negative regulation of epidermal growth factor receptor signaling pathway
positive regulation of apoptotic process
positive regulation of GTPase activity
ephrin receptor signaling pathway
focal adhesion assembly
regulation of small GTPase mediated signal transduction
positive regulation of growth hormone secretion
positive regulation of substrate adhesion-dependent cell spreading
regulation of GTP binding
positive regulation of lamellipodium morphogenesis
small GTPase mediated signal transduction
astrocyte cell migration
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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