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Proteintech
ARK5 Polyclonal Antibody
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Product Details
22723-1-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
ARK5 Fusion Protein Ag18612 (515-662 aa encoded by BC152462)
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Amount
31.5 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
Immunogen sequence: HSLSCRRKG ILKHSSKYSA GTMDPALVSP EMPTLESLSE PGVPAEGLSR SYSRPSSVIS DDSVLSSDSF DLLDLQENRP ARQRIRSCVS AENFLQIQDF EGLQNRPRPQ YLKRYRNRLA DSSFSLLTDM DDVTQVYKQA LEICSKLN (515-662 aa encoded by BC152462)
Target Information
NUAK1 (ARK5) is a serine/threonine kinase that plays a role in many cellular functions. The overexpression of this kinase has been associated with tumor invasion and metastasis (colorectal neoplasm). Acts as a regulator of cellular senescence and cellular ploidy by mediating phosphorylation of 'Ser-464' of LATS1, thereby controlling its stability. Controls cell adhesion by regulating activity of the myosin protein phosphatase 1 (PP1) complex. Acts by mediating phosphorylation of PPP1R12A subunit of myosin PP1: phosphorylated PPP1R12A then interacts with 14-3-3, leading to reduced dephosphorylation of myosin MLC2 by myosin PP1. May be involved in DNA damage response: phosphorylates p53/TP53 at 'Ser-15' and 'Ser-392' and is recruited to the CDKN1A/WAF1 promoter to participate in transcription activation by p53/TP53. May also act as a tumor malignancy-associated factor by promoting tumor invasion and metastasis under regulation and phosphorylation by AKT1. Suppresses Fas-induced apoptosis by mediating phosphorylation of CASP6, thereby suppressing the activation of the caspase and the subsequent cleavage of CFLAR. Regulates UV radiation-induced DNA damage response mediated by CDKN1A. In association with STK11, phosphorylates CDKN1A in response to UV radiation and contributes to its degradation which is necessary for optimal DNA repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: AMP-activated protein kinase family member 5; AMPK-related protein kinase 5; ARK5; NUAK family SNF1-like kinase 1; NUAK family, SNF1-like kinase, 1; Omphalocele kinase 1; unnamed protein product; ZNUAK family, SNF1-like kinase, 1
Gene Aliases: ARK5; AU014801; AW494241; B230104P22Rik; KIAA0537; NUAK1; OMPHK1; RGD1309956
UniProt ID: (Human) O60285, (Mouse) Q641K5
Entrez Gene ID: (Human) 9891, (Rat) 299694, (Mouse) 77976
nucleotide binding
p53 binding
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
kinase activity
transferase activity
metal ion binding
protein serine kinase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
protein phosphorylation
cellular response to DNA damage stimulus
cell adhesion
regulation of cell adhesion
regulation of cell proliferation
regulation of signal transduction by p53 class mediator
regulation of cellular senescence
regulation of myosin-light-chain-phosphatase activity
phosphorylation
negative regulation of TOR signaling
intracellular signal transduction
cellular response to glucose starvation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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