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Invitrogen
ARNT Polyclonal Antibody
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FIGURE: 1 / 1
ARNT Antibody (PA1-515) in ICC/IF
Immunofluorescent analysis of ARNT (green) in NRK cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with an ARNT Polyclonal Antibody (Product # PA1-515) at a dilution of 20 µg/mL in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor 488 conjugate (Product # A2... View More
Product Details
PA1-515
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C N(771) S Y N N E E F P D L T M F P P F S E(789)
Conjugate
Form
Liquid
Concentration
0.6 mg/mL
Amount
60 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-515 detects Ah receptor nuclear translocator (ARNT) from mouse and rat cells.
PA1-515 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects an ~87 kDa protein representing ARNT from Hepa 1c1c7 cell lysate. PA1-515 reacts with one other band lower than ARNT whose identity has not been established.
PA1-515 immunizing peptide corresponds to amino acid residues 771-789 from human ARNT. This sequence is completely conserved between human and mouse ARNT.
Target Information
HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. Hypoxia contributes significantly to the pathophysiology of major categories of human disease, including myocardial and cerebral ischemia, cancer, pulmonary hypertension, congenital heart disease and chronic obstructive pulmonary disease. HIF-1 is a nuclear protein involved in mammalian oxygen homeostasis. This occurs as a posttranslational modification by prolyl hydroxylation. HIF-1 is a heterodimer composed of HIF-1 alpha and HIF-1 beta subunits. Both subunits are constantly translated. However, under normoxic conditions, human HIF-1 alpha is hydroxylated at Pro402 or Pro564 by a set of HIF prolyl hydroxylases, is polyubiquinated, and eventually degraded in proteosomes. Under hypoxic conditions, the lack of hydroxylation prevents HIF degradation and increases transcriptional activity. Therefore, the concentration of HIF-1 alpha increases in the cell. In contrast, HIF-1 beta remains stable under either condition. HIF-1 beta is a series of aryl hydrocarbon receptor nuclear translocator (ARNT) gene products. Diseases associated with HIF-1 beta dysfunction include hypoxia and renal cell carcinoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: ARNT protein; Aryl hydrocarbon receptor nuclear translocator; Aryl hydrocarbon receptor nuclear translocator 1; basic helix-loop-helix transcription factor; bHLHe2; Class E basic helix-loop-helix protein 2; Dioxin receptor, nuclear translocator; hif 1; HIF-1 beta; HIF-1-beta; HIF1 beta; HIF1-beta; hypoxia inducible factor 1 subunit beta; hypoxia-inducible factor 1 beta; hypoxia-inducible factor 1, beta subunit; Hypoxia-inducible factor 1-beta; unnamed protein product
Gene Aliases: ARNT; ARNT1; BHLHE2; D3Ertd557e; Drnt; ESTM42; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; mKIAA4051; TANGO; W08714
UniProt ID: (Human) P27540, (Rat) P41739, (Mouse) P53762
Entrez Gene ID: (Human) 405, (Rat) 25242, (Mouse) 11863
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
aryl hydrocarbon receptor binding
protein homodimerization activity
sequence-specific DNA binding
protein heterodimerization activity
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
sequence-specific double-stranded DNA binding
transcription factor activity, transcription factor binding
transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding
transcription coactivator activity
signal transducer activity
aryl hydrocarbon receptor activity
transcription factor binding
enhancer binding
basic helix-loop-helix transcription factor
response to hypoxia
embryonic placenta development
positive regulation of endothelial cell proliferation
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of vascular endothelial growth factor production
cell differentiation
intracellular receptor signaling pathway
positive regulation of vascular endothelial growth factor receptor signaling pathway
positive regulation of protein sumoylation
cellular response to oxidative stress
positive regulation of erythrocyte differentiation
positive regulation of glycolytic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of hormone biosynthetic process
negative regulation of inflammatory response
intestinal epithelial structure maintenance
transcription, DNA-templated
transcription from RNA polymerase II promoter
signal transduction
response to toxic substance
regulation of transcription from RNA polymerase II promoter in response to oxidative stress
regulation of nucleic acid-templated transcription
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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