|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 3 and 189 of Human ARS2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA5-31593 targets ARS2 in IF, IHC (P), and WB applications and shows reactivity with Human samples.
The PA5-31593 immunogen is recombinant protein fragment corresponding to a region within amino acids 3 and 189 of Human ARS2.
Acts as a mediator between the cap-binding complex (CBC) and the primary microRNAs (miRNAs) processing machinery during cell proliferation. Contributes to the stability and delivery of capped primary miRNA transcripts to the primary miRNA processing complex containing DGCR8 and RNASEN, thereby playing a role in RNA-mediated gene silencing (RNAi) by miRNAs. Binds capped RNAs (m7GpppG-capped RNA); however interaction is probably mediated via its interaction with NCBP1/CBP80 component of the CBC complex. Involved in cell cycle progression at S phase. Does not directly confer arsenite resistance but rather modulates arsenic sensitivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
mRNA export through an additional cap-binding complex consisting of NCBP1 and NCBP3.
PA5-31593 was used in western blot to elucidate the roles of nuclear cap-binding protein 1-3 in mRNA biogenesis
|Gebhardt A,Habjan M,Benda C,Meiler A,Haas DA,Hein MY,Mann A,Mann M,Habermann B,Pichlmair A||Nature communications (6:null)||2015|