Invitrogen

AS160 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (29) AS160 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

AS160 Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, HeLa cells were fixed and permeabilized for detection of endogenous AS160 using anti- AS160/TBC1D4 Rabbit Polyclonal Antibody (Product # 720304, 2µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of AS160 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of AS160. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

AS160 Antibody in Western Blot (WB) — Western blot analysis was performed on Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Caco-2 (Lane 2), Hep G2 (Lane 3) HEK-293 (Lane 4), HEL 92.1.7 (Lane 5) and MOLT4 (Lane 6). The blots were probed with Anti-AS160/TBC1D4 Rabbit Polyclonal Antibody (Product # 720304, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 147 kDa band corresponding to AS160/TBC1D4 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane by wet transfer method. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

AS160 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. Caco-2 cells were transfected with AS160 siRNA and decrease in signal intensity was observed in western blot application using Anti-AS160 Polyclonal Antibody (Product # 720304). {KD}

Product Details

720304

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptides corresponding to Human TBC1D4 (aa 183-199, 936-954) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 0.1% BSA, 30% glycerol
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2633249

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Horse, Dog and Cat

Target Information

AS160 (TBC1D4) is a GTPase-activiting protein for RAB2A, RAB8A, RAB10, and RAB14. The isoform 2 version of this protein promotes the insulin-induced glucose transporter SLC2A4/GLUT4 translocation at the plasma membrane. This movement increases glucose uptake. Mutations associated with the gene cause Diabetes mellitus, non-insulin-dependent, 5 (NIDDM5).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Akt substrate of 160 kDa; AS160; DKFZp779C0666; OTTHUMP00000040818; RP11-159J2.1; RP11-159J2.3; TBC (Tre-2, BUB2, CDC16) domain-containing protein; TBC Tre-2; TBC1 domain family member 4; TBC1 domain family, member 4

Gene Aliases: AS160; KIAA0603; NIDDM5; TBC1D4

UniProt ID: (Human) O60343

Entrez Gene ID: (Human) 9882

Molecular Function: GTPase-activating protein

Function(s)

intracellular protein transport vesicle-mediated transport regulation of vesicle fusion negative regulation of vesicle fusion cellular response to insulin stimulus membrane organization activation of GTPase activity

Process(es)

GTPase activator activity Rab GTPase binding

AS160 Polyclonal Antibody

Product Details

720304

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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