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Invitrogen
ASAH2 Polyclonal Antibody
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FIGURE: 1 / 1
ASAH2 Antibody (PA5-88650) in WB
Western blot analysis of ASAH2 in extracts of various cell lines. Samples were incubated with ASAH2 Polyclonal antibody (Product # PA5-88650) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-88650
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein (or fragment).
Conjugate
Form
Liquid
Concentration
0.7 mg/mL
Amount
70 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: TATQTSPVPL TPESPLFQNF SGYHIGVGRA DCTGQVADIN LMGYGKSGQN AQGILTRLYS RAFIMAEPDG SNRTVFVSID IGMVSQRLRL EVLNRLQSKY GSLYRRDNVI LSGTHTHSGP AGYFQYTVFV IASEGFSNQT FQHMVTGILK SIDIAHTNMK PGKIFINKGN VDGVQINRSP YSYLQNPQSE RARYSSNTDK EMIVLKMVDL NGDDLGLISW FAIHPVSMNN SNHLVNSDNV GYASYLLEQE KNKGYLPGQG P; Positive Samples: 22Rv1, Mouse kidney, Rat kidney; Cellular Location: Cell membrane, Single-pass type II membrane protein
Target Information
Sphingolipids are hydrolyzed by ceramidases to yield sphingosine and fatty acids. These ceramidases are classified according to the pH range that supports their optimal activity. ASAH2 is a neutral ceramidase and key regulator of sphingolipid signaling metabolites at the cell surface, catalyzing the hydrolysis of the N-acyl linkage of ceramide at an optimal pH of 6.5-8.5. ASAH2 is a type II integral membrane protein that can be cleaved to yield a soluble secreted protein and acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger. ASAH2 is ubiquitously expressed primarily expressed with higher levels in the intestine, kidney, skeletal muscle and heart. Recent studies indicate that ASAH2 encoded neutral ceramidase is a key enzyme for the catabolism of dietary sphingolipids and regulates the levels of bioactive sphingolipid metabolites in the intestinal tract.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Acylsphingosine deacylase 2; BCDase; ceramide hydrolase; hCD; LCDase; mitochondrial ceramidase; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; N-acylsphingosine amidohydrolase 2; N-CDase; NCDase; Neutral ceramidase; neutral/alkaline ceramidase; Non-lysosomal ceramidase
Gene Aliases: AI585898; ASAH2; BCDase; HNAC1; LCDase; N-CDase; NCDase
UniProt ID: (Human) Q9NR71, (Mouse) Q9JHE3, (Rat) Q91XT9
Entrez Gene ID: (Human) 56624, (Mouse) 54447, (Rat) 114104
lipid metabolic process
sphingolipid metabolic process
apoptotic process
cell proliferation
response to organic substance
sphingosine metabolic process
ceramide metabolic process
regulation of mitotic cell cycle
long-chain fatty acid biosynthetic process
lipid digestion
sphingosine biosynthetic process
ceramide biosynthetic process
ceramide catabolic process
cellular response to cytokine stimulus
negative regulation of apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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