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FIGURE: 1 / 1
ASCC2 Antibody (PA5-89440) in WB
Western blot analysis of ASCC2 in extracts of various cell lines. Samples were incubated with ASCC2 Polyclonal antibody (Product # PA5-89440) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Product Details
PA5-89440
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 488-757 of human ASCC2 (NP_1155802)
Conjugate
Form
Liquid
Concentration
0.36 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805572
Product Specific Information
Immunogen sequence: LACLEYYHYD PEQVINNILE ERLAPTLSQL DRNLDREMKP DPTPLLTSRH NVFQNDEFDV FSRDSVDLSR VHKGKSTRKE ENTRSLLNDK RAVAAQRQRY EQYSVVVEEV PLQPGESLPY HSVYYEDEYD DTYDGNQVGA NDADSDDELI SRRPFTIPQV LRTKVPREGQ EEDDDDEEDD ADEEAPKPDH FVQDPAVLRE KAEARRMAFL AKKGYRHDSS TAVAGSPRGH GQSRETTQER RKKEANKATR ANHNRRTMAD RKRSKGMIPS; Positive Samples: HT-29, HepG2, A-431, HeLa, 293T, Mouse eye, Rat pancreas
Target Information
Plays a role in DNA damage repair as component of the ASCC complex. Recruits ASCC3 and ALKBH3 to sites of DNA damage by binding to polyubiquitinated proteins that have 'Lys-63'-linked polyubiquitin chains (PubMed:29144457). Part of the ASC-1 complex that enhances NF-kappa-B, SRF and AP1 transactivation (PubMed:12077347). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Activating signal cointegrator 1 complex subunit 2; ASC-1 complex subunit P100; Trip4 complex subunit p100
Gene Aliases: 1700011I11Rik; 2610034L15Rik; AI482016; ASC1P100; ASCC2; AW046480; p100; RGD1561422; RQT3
UniProt ID: (Human) Q9H1I8, (Mouse) Q91WR3
Entrez Gene ID: (Human) 84164, (Mouse) 75452, (Rat) 498402
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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