Invitrogen

ASCL1 Polyclonal Antibody

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View all (24) ASCL1 antibodies

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Datasheet
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Datasheet
Protocols
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Datasheet
Protocols
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Product Details

PA5-77868

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:100-1:1,000
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Product Specifications

Species Reactivity

Dog, Human, Mouse, Rat

Published species

C. elegans, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human ASCL1. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.65 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2735483

Product Specific Information

Positive Control: U87-MG, SK-N-SH, IMR32, SK-N-AS, SH-SY5Y, SH-SY5Y nuclear extract, PC-12, Rat2

Predicted Reactivity: Mouse (84%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ASCL1 (also known as ASH1) is a basic helix-loop-helix transcription factor that is required for early development of the nervous system. Expressed in fetal brain, ASCL1 is essential for the proper development of autonomic neurons and for the survival of subsets of autonomic neurons. ASCL1 interaction with MEF-2A may regulate the expression of specific genes that are critical for the formation of distinct neuronal circuits within the central nervous system. The high level of ASCL1 expression in neuroendocrine tumors, such as medullary thyroid cancer, small cell lung cancer and lung cancer with neuroendocrine features may provide a useful marker for cancers with neuroendocrine features. Mapping to human chromosome 12, the ASCL1 gene contains a trinucleotide repeat region, making this locus a candidate for inherited disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: achaete scute protein; achaete-scute complex homolog 1; achaete-scute complex homolog-like 1; achaete-scute complex-like 1; achaete-scute complex-like protein 1; Achaete-scute homolog 1; ASH-1; bHLHa46; Class A basic helix-loop-helix protein 46; KMT2H; mammalian achaete scute homolog 1; mASH-1

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Gene Aliases: AI225900; ASCL1; ASH1; BHLHA46; HASH1; Mash-1; MASH1

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UniProt ID: (Human) P50553, (Rat) P19359, (Mouse) Q02067

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Entrez Gene ID: (Dog) 482628, (Human) 429, (Rat) 64186, (Mouse) 17172

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, transcription factor binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding protein binding protein homodimerization activity bHLH transcription factor binding E-box binding double-stranded DNA binding sequence-specific DNA binding protein dimerization activity basic helix-loop-helix transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter neuron migration noradrenergic neuron development noradrenergic neuron fate commitment transcription, DNA-templated Notch signaling pathway regulation of mitotic cell cycle neuroblast fate determination neuroblast proliferation response to lithium ion regulation of gene expression oligodendrocyte development spinal cord association neuron differentiation spinal cord oligodendrocyte cell fate specification vestibular nucleus development cerebral cortex GABAergic interneuron differentiation commitment of neuronal cell to specific neuron type in forebrain central nervous system neuron development cerebral cortex development neurogenesis regulation of epithelial cell differentiation response to retinoic acid negative regulation of apoptotic process positive regulation of neuron apoptotic process negative regulation of neuron differentiation positive regulation of neuron differentiation positive regulation of Notch signaling pathway positive regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell maturation sympathetic nervous system development neuron fate specification musculoskeletal movement, spinal reflex action response to folic acid subpallium neuron fate commitment regulation of timing of subpallium neuron differentiation olfactory pit development lung epithelial cell differentiation ventral spinal cord interneuron fate commitment lung neuroendocrine cell differentiation stomach neuroendocrine cell differentiation carotid body glomus cell differentiation adrenal chromaffin cell differentiation sympathetic ganglion development response to epidermal growth factor cellular response to magnetism positive regulation of neural precursor cell proliferation regulation of transcription from RNA polymerase II promoter pattern specification process nervous system development regulation of Notch signaling pathway glial cell differentiation spinal cord oligodendrocyte cell differentiation oligodendrocyte cell fate commitment forebrain neuron differentiation cell differentiation neuron differentiation regulation of cell proliferation neuron fate commitment neuron development generation of neurons oligodendrocyte differentiation regulation of neurogenesis positive regulation of neurogenesis regulation of transcription, DNA-templated transcription from RNA polymerase II promoter multicellular organism development enteric nervous system development parasympathetic nervous system development
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