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Invitrogen
ASIC2 Polyclonal Antibody
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FIGURE: 1 / 6
ASIC2 Antibody (PA5-143911) in ICC/IF
Immunocytochemistry analysis of ASIC2 in A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in ASIC2 Polyclonal antibody (Product # PA5-143911) using a dilution of 2 μg/mL. DyLight®488 conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More
Product Details
PA5-143911
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human ACCN1/ASIC2 recombinant protein (Position: H27-C512).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human placenta tissue, human PC-3 whole cell, human A549 whole cell, human U2OS whole cell, rat brain tissue, rat liver tissue, rat ovarian tissue, mouse brain tissue, mouse liver tissue, mouse ovarian tissue. ICC/IF: A431 cell. Flow: A549 cell, PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
ACCN1 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this protein may play a role in neurotransmission. In addition, a heteromeric association between this member and ACCN3 (variant 1) has been observed to co-assemble into proton-gated channels sensitive to gadolinium.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: acid sensing (proton gated) ion channel 2; Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive brain sodium channel 2; Amiloride-sensitive cation channel 1, neuronal; amiloride-sensitive cation channel 1, neuronal (degenerin); Amiloride-sensitive cation channel neuronal 1; ASIC2; BNC1; brain Na+ channel-1; Brain sodium channel 1; degenerin; Mammalian degenerin homolog; MDEG; neuronal amiloride-sensitive cation channel 1; unnamed protein product
Gene Aliases: ACCN; ACCN1; ACIC2; ASIC2; ASIC2a; BNaC1; BNaC1a; BNC1; BNC1k; hBNaC1; MDEG; MDEG1; MDEG2
UniProt ID: (Human) Q16515, (Rat) Q62962, (Mouse) Q925H0
Entrez Gene ID: (Human) 40, (Rat) 25364, (Mouse) 11418
regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback
ion transport
cation transport
sodium ion transport
synapse assembly
phototransduction
sensory perception of sound
response to mechanical stimulus
response to acidic pH
regulation of vasoconstriction
ion transmembrane transport
regulation of ion transmembrane transport
protein localization to synapse
sodium ion transmembrane transport
regulation of membrane potential
negative regulation of apoptotic process
sensory perception of sour taste
detection of mechanical stimulus involved in sensory perception
establishment of localization in cell
positive regulation of synapse assembly
cellular response to xenobiotic stimulus
cellular response to acidic pH
regulation of postsynapse assembly
regulation of gene expression
regulation of blood coagulation
cellular response to drug
cation transmembrane transport
positive regulation of cation channel activity
transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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