Invitrogen

ASIC2 Polyclonal Antibody

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

ASIC2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human brain tissue using an ACCN1 polyclonal antibody (Product # PA5-26222), followed by HRP-conjugated secondary antibody and DAB staining.

ASIC2 Antibody in Western Blot (WB) — Western blot analysis using an ACCN1 polyclonal antibody (Product # PA5-26222) in NCI-H460 cell lysates (35 µg per lane).

ASIC2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of WiDr cells using an ACCN1 polyclonal antibody (Product # PA5-26222) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

ASIC2 Antibody in Immunohistochemistry (IHC) — Figure 5 Summary of standard immunohistochemical and TMA score results for ASIC1, ASIC2, TRPV1 and TRPV4 on ( a ) SCC, ( b ) BCC, ( c ) NCN and ( d ) MM. ++/green bar: strong positive staining with >80% of cells positive and/or staining intensity is high; +/blue bar: 20-80% of cells show a weak positive/partial positive reaction; -/red bar: <20% of cells with weak staining (=negative reaction). NCN and MM are subdivided into epidermal and dermal portions. Numbers in bars represent the occurrence of the particular score. Superscript numbers give additional information: (1) some samples showed a decreasing expression in deeper dermal tumor tissue. (2) Single tumor cells are stained strong positive, others appear negative, resulting in an overall partial positive score (+). Overall trend is indicated by a green, white or red box with the number of samples investigated; green box: general positive reactions; white box: mixed reactions; red box: mainly negative reactions. For additional information on the individual TMA scores, see Supplementary Tables S2-S5 .

Product Details

PA5-26222

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

Flow Cytometry (Flow)

1:10-1:50

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

KLH conjugated synthetic peptide between 120-148 amino acids from the central region of human ACCN1

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Purification

Protein A, Antigen affinity chromatography

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2543722

Product Specific Information

This antibody is predicted to react with mouse and rat based on sequence homology.

Target Information

ACCN1 encodes a member of the degenerin/epithelial sodium channel (DEG/ENaC) superfamily. The members of this family are amiloride-sensitive sodium channels that contain intracellular N and C termini, 2 hydrophobic transmembrane regions, and a large extracellular loop, which has many cysteine residues with conserved spacing. The member encoded by this protein may play a role in neurotransmission. In addition, a heteromeric association between this member and ACCN3 (variant 1) has been observed to co-assemble into proton-gated channels sensitive to gadolinium.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression Profiles of ASIC1/2 and TRPV1/4 in Common Skin Tumors.

International journal of molecular sciences

Ackermann K,Wallner S,Brochhausen C,Schreml S

Published figure using ASIC2 polyclonal antibody (Product # PA5-26222) in Immunohistochemistry

Wed Jun 02 00:00:00 EDT 2021

pH sensing in skin tumors: Methods to study the involvement of GPCRs, acid-sensing ion channels and transient receptor potential vanilloid channels.

Experimental dermatology

Stolwijk JA,Sauer L,Ackermann K,Nassios A,Aung T,Haerteis S,Bäumner AJ,Wegener J,Schreml S

Published figure using ASIC2 polyclonal antibody (Product # PA5-26222) in Immunohistochemistry

Sun Nov 01 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: acid sensing (proton gated) ion channel 2; acid sensing ion channel 2; acid-sensing (proton-gated) ion channel 2; Acid-sensing ion channel 2; Amiloride-sensitive brain sodium channel; Amiloride-sensitive cation channel 1, neuronal; Amiloride-sensitive cation channel neuronal 1; ASIC2; BNC1; Brain sodium channel 1; Mammalian degenerin homolog; MDEG; neuronal amiloride-sensitive cation channel 1

Gene Aliases: ACCN; ACCN1; ASIC2; ASIC2a; BNAC1; BNC1; hBNaC1; MDEG

UniProt ID: (Human) Q16515

Entrez Gene ID: (Human) 40

Molecular Function: ion channel

Function(s)

regulation of systemic arterial blood pressure by aortic arch baroreceptor feedback chemical synaptic transmission central nervous system development peripheral nervous system development phototransduction sensory perception of sound response to acidic pH regulation of gene expression monovalent inorganic cation transport regulation of vasoconstriction regulation of blood coagulation ion transmembrane transport regulation of ion transmembrane transport protein localization to synapse sodium ion transmembrane transport regulation of membrane potential negative regulation of apoptotic process sensory perception of sour taste detection of mechanical stimulus involved in sensory perception positive regulation of synapse assembly

Process(es)

protein binding ligand-gated sodium channel activity ion gated channel activity acid-sensing ion channel activity