Immunofluorescent analysis of ASIC3 using an ASIC3 polyclonal antibody (Product # PA1-27981). ASIC3 expression compared to other nociceptive ion channels. DRG sections double-labeled for ASIC3 (red) and either P2X3 (green, A) or TRPV1 (green, B); double-labeled cells are yellow or orange. Circle charts show the relative distribution of the three receptors among all DRG neurons. ASIC3 and P2X3 expression overlapped in only 4% of neurons, while some 35% of ASIC3-positive neurons also express TRPV1. The rare cells positive for both ASIC3 and P2X3 were large (eg. cell at lower left in A). 1406 cells counted for A; 995 for B. C, D, electrophysiological recordings reveal a qualitatively similar expression pattern for currents conforming to ASIC3, P2X3, or TRPV1. The cell that generated the currents in C counts as a co-expressor of ASIC3 and P2X3, fitting into the yellow bin in the circle plot. The cell in D is a co-expressor of ASIC3 and TRPV1. Cells were counted positive if they had at least 0.3 nA of current in response to pH 6.8 (ASIC3+), 50µM ATP (P2X3+; transient current only), or 1µM capsaicin (TRPV1). 182 cells recorded for C; 169 for D.
|Tested species reactivity||Rat|
|Host / Isotype||Guinea pig / IgG|
|Immunogen||synthetic peptide TASLDPDDFDPEPSDPLGSP, corresponding to amino acids 285-304 of extracellular domain of Rat ASIC3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:100|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-27981 detects ASIC3 from rat samples.
PA1-27981 has been successfully used in immunocytochemistry, immunohistochemistry (frozen tissue) applications.
The PA1-27981 immunogen is: Synthetic peptide: TASLDPDDFDPEPSDPLGSP, corresponding to amino acids 285-304 of extracellular domain of Rat ASIC3.
subunit that forms a proton gated Na(+) channel; may play a role in prolonged pain perception.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.