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ASL Antibody (PA5-22052) in ICC/IF
ASL Polyclonal Antibody detects ASL protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ASL protein stained by ASL Polyclonal Antibody (Product # PA5-22052) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-22052
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 86 and 325 (P04424) of ASL
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_11153094
Product Specific Information
Recommended positive controls: HeLa, Molt-4.
Predicted reactivity: Mouse (86%), Rat (83%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ASL encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: argininosuccinase; Argininosuccinate lyase; Arginosuccinase; ASAL
Gene Aliases: ASAL; ASL
UniProt ID: (Human) P04424
Entrez Gene ID: (Human) 435
Performance Guarantee
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