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ASNS Polyclonal Antibody

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ASNS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 14681-1-AP (ASNS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ASNS Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 14681-1-AP (ASNS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ASNS Antibody in Immunoprecipitation (IP) — IP result of anti-ASNS (IP:14681-1-AP, 4ug; Detection:14681-1-AP 1:500) with HeLa cells lysate 1200ug.

Product Details

14681-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 6 publications 6 publications
Immunocytochemistry (ICC/IF)	1:20-1:200	-
Immunoprecipitation (IP)	0.5-4.0 µg	-
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ASNS Fusion Protein Ag6393 (247-561 aa encoded by BC014621) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: LMTDRRIGC LLSGGLDSSL VAATLLKQLK EAQVQYPLQT FAIGMEDSPD LLAARKVADH IGSEHYEVLF NSEEGIQALD EVIFSLETYD ITTVRASVGM YLISKYIRKN TDSVVIFSGE GSDELTQGYI YFHKAPSPEK AEEESERLLR ELYLFDVLRA DRTTAAHGLE LRVPFLDHRF SSYYLSLPPE MRIPKNGIEK HLLRETFEDS NLIPKEILWR PKEAFSDGIT SVKNSWFKIL QEYVEHQVDD AMMANAAQKF PFNTPKTKEG YYYRQVFERH YPGRADWLSH YWMPKWINAT DPSARTLTHY KSAVKA (247-561 aa encoded by BC014621)

Target Information

ASNS (asparagine synthetase [glutamine-hydrolyzing]) is involved in the subpathway that synthesizes f L-asparagine from L-aspartate. Mutations affecting the gene can result in asparagine synthetase deficiency (ASNSD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction.

Communications biology

Cheng CT,Qi Y,Wang YC,Chi KK,Chung Y,Ouyang C,Chen YR,Oh ME,Sheng X,Tang Y,Liu YR,Lin HH,Kuo CY,Schones D,Vidal CM,Chu JC,Wang HJ,Chen YH,Miller KM,Chu P,Yen Y,Jiang L,Kung HJ,Ann DK

Sat Jan 11 00:00:00 EST 2020

Arginine Deprivation Inhibits the Warburg Effect and Upregulates Glutamine Anaplerosis and Serine Biosynthesis in ASS1-Deficient Cancers.

Cell reports

Kremer JC,Prudner BC,Lange SES,Bean GR,Schultze MB,Brashears CB,Radyk MD,Redlich N,Tzeng SC,Kami K,Shelton L,Li A,Morgan Z,Bomalaski JS,Tsukamoto T,McConathy J,Michel LS,Held JM,Van Tine BA

Tue Jan 24 00:00:00 EST 2017

Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.

Cancer gene therapy

Xu Y,Lv F,Zhu X,Wu Y,Shen X

Thu Sep 01 00:00:00 EDT 2016

Transcriptome Analysis of Piperlongumine-Treated Human Pancreatic Cancer Cells Reveals Involvement of Oxidative Stress and Endoplasmic Reticulum Stress Pathways.

Journal of medicinal food

Dhillon H,Mamidi S,McClean P,Reindl KM

Wed Jun 01 00:00:00 EDT 2016

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Biotechnology and applied biochemistry

Li H,Zhou F,Du W,Dou J,Xu Y,Gao W,Chen G,Zuo X,Sun L,Zhang X,Yang S

Sun May 01 00:00:00 EDT 2016

Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor.

Nature communications

Krall AS,Xu S,Graeber TG,Braas D,Christofk HR

Fri Apr 29 00:00:00 EDT 2016

Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.

Chemical biology & drug design

Yang H,He X,Zheng Y,Feng W,Xia X,Yu X,Lin Z

Sat Nov 01 00:00:00 EDT 2014

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 EDT 2014

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; TS11 cell cycle control protein

Gene Aliases: ASNS; ASNSD; TS11

UniProt ID: (Human) P08243

Entrez Gene ID: (Human) 440

Molecular Function: ligase

Function(s)

liver development asparagine biosynthetic process glutamine metabolic process cellular amino acid biosynthetic process response to light stimulus response to mechanical stimulus response to toxic substance response to methotrexate response to follicle-stimulating hormone cellular response to hormone stimulus PERK-mediated unfolded protein response cellular response to glucose starvation negative regulation of apoptotic process response to amino acid positive regulation of mitotic cell cycle L-asparagine biosynthetic process

Process(es)

asparagine synthase (glutamine-hydrolyzing) activity protein binding ATP binding protein homodimerization activity cofactor binding

ASNS Polyclonal Antibody

Product Details

14681-1-AP

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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