Proteintech

ASNS Polyclonal Antibody

ASNS Antibody in Immunocytochemistry (ICC/IF)

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ASNS Antibody (14681-1-AP) in ICC/IF

Immunofluorescent analysis of ( -20°C Ethanol ) fixed SH-SY5Y cells using 14681-1-AP (ASNS antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ASNS Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ASNS Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

14681-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 34 publications 34 publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ASNS Fusion Protein Ag6393 (247-561 aa encoded by BC014621)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.27 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: LMTDRRIGC LLSGGLDSSL VAATLLKQLK EAQVQYPLQT FAIGMEDSPD LLAARKVADH IGSEHYEVLF NSEEGIQALD EVIFSLETYD ITTVRASVGM YLISKYIRKN TDSVVIFSGE GSDELTQGYI YFHKAPSPEK AEEESERLLR ELYLFDVLRA DRTTAAHGLE LRVPFLDHRF SSYYLSLPPE MRIPKNGIEK HLLRETFEDS NLIPKEILWR PKEAFSDGIT SVKNSWFKIL QEYVEHQVDD AMMANAAQKF PFNTPKTKEG YYYRQVFERH YPGRADWLSH YWMPKWINAT DPSARTLTHY KSAVKA (247-561 aa encoded by BC014621)

Target Information

ASNS (asparagine synthetase [glutamine-hydrolyzing]) is involved in the subpathway that synthesizes f L-asparagine from L-aspartate. Mutations affecting the gene can result in asparagine synthetase deficiency (ASNSD).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Role of asparagine biosynthesis pathway in Siniperca chuatsi rhabdovirus proliferation.

Frontiers in microbiology

Li F,Fu X,Luo X,Lin Q,Liang H,Niu Y,Liu L,Li N

Tue Apr 18 00:00:00 UTC 2023

Arginine limitation causes a directed DNA sequence evolution response in colorectal cancer cells.

bioRxiv : the preprint server for biology

Hsu DJ,Gao J,Yamaguchi N,Pinzaru A,Mandayam N,Liberti M,Heissel S,Alwaseem H,Tavazoie S,Tavazoie SF

Mon Jan 02 00:00:00 UTC 2023

Arginine limitation drives a directed codon-dependent DNA sequence evolution response in colorectal cancer cells.

Science advances

Hsu DJ,Gao J,Yamaguchi N,Pinzaru A,Wu Q,Mandayam N,Liberti M,Heissel S,Alwaseem H,Tavazoie S,Tavazoie SF

Fri Jan 06 00:00:00 UTC 2023

A coordinated multiorgan metabolic response contributes to human mitochondrial myopathy.

EMBO molecular medicine

Southwell N,Primiano G,Nadkarni V,Attarwala N,Beattie E,Miller D,Alam S,Liparulo I,Shurubor YI,Valentino ML,Carelli V,Servidei S,Gross SS,Manfredi G,Chen Q,D'Aurelio M

Mon Jul 10 00:00:00 UTC 2023

Transcriptional and metabolic remodeling in clear cell renal cell carcinoma caused by ATF4 activation and the integrated stress response (ISR).

Molecular carcinogenesis

van der Mijn JC,Chen Q,Laursen KB,Khani F,Wang X,Dorsaint P,Sboner A,Gross SS,Nanus DM,Gudas LJ

Thu Sep 01 00:00:00 UTC 2022

Transcriptional and metabolic remodeling in clear cell renal cell carcinoma caused by ATF4 activation and the integrated stress response (ISR).

Molecular carcinogenesis

van der Mijn JC,Chen Q,Laursen KB,Khani F,Wang X,Dorsaint P,Sboner A,Gross SS,Nanus DM,Gudas LJ

Thu Sep 01 00:00:00 UTC 2022

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Molecular cell

Jiang B,Zhang J,Zhao G,Liu M,Hu J,Lin F,Wang J,Zhao W,Ma H,Zhang C,Wu C,Yao L,Liu Q,Chen X,Cao Y,Zheng Y,Zhang C,Han A,Lin D,Li Q

Thu May 19 00:00:00 UTC 2022

Asparagine bioavailability regulates the translation of MYC oncogene.

Oncogene

Srivastava S,Jiang J,Misra J,Seim G,Staschke KA,Zhong M,Zhou L,Liu Y,Chen C,Davé U,Kapur R,Batra S,Zhang C,Zhou J,Fan J,Wek RC,Zhang J

Sat Oct 01 00:00:00 UTC 2022

Tumoral microenvironment prevents de novo asparagine biosynthesis in B cell lymphoma, regardless of ASNS expression.

Science advances

Grima-Reyes M,Vandenberghe A,Nemazanyy I,Meola P,Paul R,Reverso-Meinietti J,Martinez-Turtos A,Nottet N,Chan WK,Lorenzi PL,Marchetti S,Ricci JE,Chiche J

Fri Jul 08 00:00:00 UTC 2022

Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.

Frontiers in neurology

Höllerhage M,Stepath M,Kohl M,Pfeiffer K,Chua OWH,Duan L,Hopfner F,Eisenacher M,Marcus K,Höglinger GU

Sat Apr 30 00:00:00 UTC 2022

UCP2 silencing restrains leukemia cell proliferation through glutamine metabolic remodeling.

Frontiers in immunology

Sancerni T,Renoult O,Luby A,Caradeuc C,Lenoir V,Croyal M,Ransy C,Aguilar E,Postic C,Bertho G,Dentin R,Prip-Buus C,Pecqueur C,Alves-Guerra MC

Tue Oct 25 00:00:00 UTC 2022

CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response.

Cell death & disease

Ruan Y,Hu J,Che Y,Liu Y,Luo Z,Cheng J,Han Q,He H,Zhou Q

Wed Feb 16 00:00:00 UTC 2022

Cellular and molecular characterization of two novel asparagine synthetase gene mutations linked to asparagine synthetase deficiency.

The Journal of biological chemistry

Staklinski SJ,Chang MC,Yu F,Collins Ruff K,Franz DN,Qian Z,Bloom LB,Merritt ME,McKenna R,Kilberg MS

Thu Sep 01 00:00:00 UTC 2022

Cellular and molecular characterization of two novel asparagine synthetase gene mutations linked to asparagine synthetase deficiency.

The Journal of biological chemistry

Staklinski SJ,Chang MC,Yu F,Collins Ruff K,Franz DN,Qian Z,Bloom LB,Merritt ME,McKenna R,Kilberg MS

Thu Sep 01 00:00:00 UTC 2022

Asparagine Synthetase-Mediated l-Asparagine Metabolism Disorder Promotes the Perineural Invasion of Oral Squamous Cell Carcinoma.

Frontiers in oncology

Fu Y,Ding L,Yang X,Ding Z,Huang X,Zhang L,Chen S,Hu Q,Ni Y

Tue Mar 30 00:00:00 UTC 2021

A Novel Ferroptosis-Based Molecular Signature Associated with Biochemical Recurrence-Free Survival and Tumor Immune Microenvironment of Prostate Cancer.

Frontiers in cell and developmental biology

Ke ZB,You Q,Sun JB,Zhu JM,Li XD,Chen DN,Su L,Zheng QS,Wei Y,Xue XY,Xu N

Tue Jan 25 00:00:00 UTC 2022

Enhanced Antitumor Effect in Liver Cancer by Amino Acid Depletion-Induced Oxidative Stress.

Frontiers in oncology

Okuda K,Umemura A,Kataoka S,Yano K,Takahashi A,Okishio S,Taketani H,Seko Y,Nishikawa T,Yamaguchi K,Moriguchi M,Nakagawa H,Liu Y,Mitsumoto Y,Kanbara Y,Shima T,Okanoue T,Itoh Y

Sat Nov 20 00:00:00 UTC 2021

Therapeutic Assessment of Targeting ASNS Combined with l-Asparaginase Treatment in Solid Tumors and Investigation of Resistance Mechanisms.

ACS pharmacology & translational science

Apfel V,Begue D,Cordo' V,Holzer L,Martinuzzi L,Buhles A,Kerr G,Barbosa I,Naumann U,Piquet M,Ruddy D,Weiss A,Ferretti S,Almeida R,Bonenfant D,Tordella L,Galli GG

Fri Feb 12 00:00:00 UTC 2021

Asparagine couples mitochondrial respiration to ATF4 activity and tumor growth.

Cell metabolism

Krall AS,Mullen PJ,Surjono F,Momcilovic M,Schmid EW,Halbrook CJ,Thambundit A,Mittelman SD,Lyssiotis CA,Shackelford DB,Knott SRV,Christofk HR

Tue May 04 00:00:00 UTC 2021

Adaptation of the heart to Frataxin depletion: Evidence that integrated stress response can predominate over mTORC1 activation.

Human molecular genetics

Vásquez-Trincado C,Patel M,Sivaramakrishnan A,Bekeová C,Anderson-Pullinger L,Wang N,Tang HY,Seifert EL

Wed Sep 22 00:00:00 UTC 2021

Canagliflozin Improves Liver Function in Rats by Upregulating Asparagine Synthetase.

Pharmacology

Wang S,Ding Y,Dong R,Wang H,Yin L,Meng S

Wed Jan 26 00:00:00 UTC 2022

Glutamine depletion regulates Slug to promote EMT and metastasis in pancreatic cancer.

The Journal of experimental medicine

Recouvreux MV,Moldenhauer MR,Galenkamp KMO,Jung M,James B,Zhang Y,Lowy A,Bagchi A,Commisso C

Mon Sep 07 00:00:00 UTC 2020

Glutamine and asparagine activate mTORC1 independently of Rag GTPases.

The Journal of biological chemistry

Meng D,Yang Q,Wang H,Melick CH,Navlani R,Frank AR,Jewell JL

Fri Mar 06 00:00:00 UTC 2020

JMJD2B-induced amino acid alterations enhance the survival of colorectal cancer cells under glucose-deprivation via autophagy.

Theranostics

Tan J,Wang HL,Yang J,Liu QQ,Li CM,Wang YQ,Fu LN,Gao QY,Chen YX,Fang JY

Mon Aug 02 00:00:00 UTC 2021

ZBTB1 Regulates Asparagine Synthesis and Leukemia Cell Response to L-Asparaginase.

Cell metabolism

Williams RT,Guarecuco R,Gates LA,Barrows D,Passarelli MC,Carey B,Baudrier L,Jeewajee S,La K,Prizer B,Malik S,Garcia-Bermudez J,Zhu XG,Cantor J,Molina H,Carroll T,Roeder RG,Abdel-Wahab O,Allis CD,Birsoy K

Tue Apr 07 00:00:00 UTC 2020

p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival.

Nature communications

Deng L,Yao P,Li L,Ji F,Zhao S,Xu C,Lan X,Jiang P

Thu Apr 09 00:00:00 UTC 2020

Suppression of asparagine synthetase enhances the antitumor potency of ART and artemalogue SOMCL-14-221 in non-small cell lung cancer.

Cancer letters

Xiao R,Ding C,Zhu H,Liu X,Gao J,Liu Q,Lu D,Zhang N,Zhang A,Zhou H

Fri Apr 10 00:00:00 UTC 2020

Promoter demethylation of the asparagine synthetase gene is required for ATF4-dependent adaptation to asparagine depletion.

The Journal of biological chemistry

Jiang J,Srivastava S,Seim G,Pavlova NN,King B,Zou L,Zhang C,Zhong M,Feng H,Kapur R,Wek RC,Fan J,Zhang J

Fri Dec 06 00:00:00 UTC 2019

Liver-Type Glutaminase GLS2 Is a Druggable Metabolic Node in Luminal-Subtype Breast Cancer.

Cell reports

Lukey MJ,Cluntun AA,Katt WP,Lin MJ,Druso JE,Ramachandran S,Erickson JW,Le HH,Wang ZE,Blank B,Greene KS,Cerione RA

Tue Oct 01 00:00:00 UTC 2019

Asparagine Is a Critical Limiting Metabolite for Vaccinia Virus Protein Synthesis during Glutamine Deprivation.

Journal of virology

Pant A,Cao S,Yang Z

Mon Jul 01 00:00:00 UTC 2019

Amino acids signatures of distance-related surgical margins of oral squamous cell carcinoma.

EBioMedicine

Yang XH,Zhang XX,Jing Y,Ding L,Fu Y,Wang S,Hu SQ,Zhang L,Huang XF,Ni YH,Hu QG

Tue Oct 01 00:00:00 UTC 2019

Asparagine Deprivation Causes a Reversible Inhibition of Human Cytomegalovirus Acute Virus Replication.

mBio

Lee CH,Griffiths S,Digard P,Pham N,Auer M,Haas J,Grey F

Tue Oct 08 00:00:00 UTC 2019

The landscape of cancer cell line metabolism.

Nature medicine

Li H,Ning S,Ghandi M,Kryukov GV,Gopal S,Deik A,Souza A,Pierce K,Keskula P,Hernandez D,Ann J,Shkoza D,Apfel V,Zou Y,Vazquez F,Barretina J,Pagliarini RA,Galli GG,Root DE,Hahn WC,Tsherniak A,Giannakis M,Schreiber SL,Clish CB,Garraway LA,Sellers WR

Wed May 01 00:00:00 UTC 2019

The landscape of cancer cell line metabolism.

Nature medicine

Wed May 01 00:00:00 UTC 2019

Autophagic reliance promotes metabolic reprogramming in oncogenic KRAS-driven tumorigenesis.

Autophagy

Lin HH,Chung Y,Cheng CT,Ouyang C,Fu Y,Kuo CY,Chi KK,Sadeghi M,Chu P,Kung HJ,Li CF,Limesand KH,Ann DK

Tue Oct 15 00:00:00 UTC 2019

Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction.

Communications biology

Cheng CT,Qi Y,Wang YC,Chi KK,Chung Y,Ouyang C,Chen YR,Oh ME,Sheng X,Tang Y,Liu YR,Lin HH,Kuo CY,Schones D,Vidal CM,Chu JC,Wang HJ,Chen YH,Miller KM,Chu P,Yen Y,Jiang L,Kung HJ,Ann DK

Sat Jan 11 00:00:00 UTC 2020

Arginine starvation kills tumor cells through aspartate exhaustion and mitochondrial dysfunction.

Communications biology

Cheng CT,Qi Y,Wang YC,Chi KK,Chung Y,Ouyang C,Chen YR,Oh ME,Sheng X,Tang Y,Liu YR,Lin HH,Kuo CY,Schones D,Vidal CM,Chu JC,Wang HJ,Chen YH,Miller KM,Chu P,Yen Y,Jiang L,Kung HJ,Ann DK

Sat Jan 11 00:00:00 UTC 2020

Arginine Deprivation Inhibits the Warburg Effect and Upregulates Glutamine Anaplerosis and Serine Biosynthesis in ASS1-Deficient Cancers.

Cell reports

Kremer JC,Prudner BC,Lange SES,Bean GR,Schultze MB,Brashears CB,Radyk MD,Redlich N,Tzeng SC,Kami K,Shelton L,Li A,Morgan Z,Bomalaski JS,Tsukamoto T,McConathy J,Michel LS,Held JM,Van Tine BA

Tue Jan 24 00:00:00 UTC 2017

Arginine Deprivation Inhibits the Warburg Effect and Upregulates Glutamine Anaplerosis and Serine Biosynthesis in ASS1-Deficient Cancers.

Cell reports

Kremer JC,Prudner BC,Lange SES,Bean GR,Schultze MB,Brashears CB,Radyk MD,Redlich N,Tzeng SC,Kami K,Shelton L,Li A,Morgan Z,Bomalaski JS,Tsukamoto T,McConathy J,Michel LS,Held JM,Van Tine BA

Tue Jan 24 00:00:00 UTC 2017

Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.

Cancer gene therapy

Xu Y,Lv F,Zhu X,Wu Y,Shen X

Thu Sep 01 00:00:00 UTC 2016

Loss of asparagine synthetase suppresses the growth of human lung cancer cells by arresting cell cycle at G0/G1 phase.

Cancer gene therapy

Xu Y,Lv F,Zhu X,Wu Y,Shen X

Thu Sep 01 00:00:00 UTC 2016

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Biotechnology and applied biochemistry

Li H,Zhou F,Du W,Dou J,Xu Y,Gao W,Chen G,Zuo X,Sun L,Zhang X,Yang S

Sun May 01 00:00:00 UTC 2016

Knockdown of asparagine synthetase by RNAi suppresses cell growth in human melanoma cells and epidermoid carcinoma cells.

Biotechnology and applied biochemistry

Li H,Zhou F,Du W,Dou J,Xu Y,Gao W,Chen G,Zuo X,Sun L,Zhang X,Yang S

Sun May 01 00:00:00 UTC 2016

Transcriptome Analysis of Piperlongumine-Treated Human Pancreatic Cancer Cells Reveals Involvement of Oxidative Stress and Endoplasmic Reticulum Stress Pathways.

Journal of medicinal food

Dhillon H,Mamidi S,McClean P,Reindl KM

Wed Jun 01 00:00:00 UTC 2016

Transcriptome Analysis of Piperlongumine-Treated Human Pancreatic Cancer Cells Reveals Involvement of Oxidative Stress and Endoplasmic Reticulum Stress Pathways.

Journal of medicinal food

Dhillon H,Mamidi S,McClean P,Reindl KM

Wed Jun 01 00:00:00 UTC 2016

Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor.

Nature communications

Krall AS,Xu S,Graeber TG,Braas D,Christofk HR

Fri Apr 29 00:00:00 UTC 2016

Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor.

Nature communications

Krall AS,Xu S,Graeber TG,Braas D,Christofk HR

Fri Apr 29 00:00:00 UTC 2016

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 UTC 2014

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 UTC 2014

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 UTC 2014

Asparagine plays a critical role in regulating cellular adaptation to glutamine depletion.

Molecular cell

Zhang J,Fan J,Venneti S,Cross JR,Takagi T,Bhinder B,Djaballah H,Kanai M,Cheng EH,Judkins AR,Pawel B,Baggs J,Cherry S,Rabinowitz JD,Thompson CB

Thu Oct 23 00:00:00 UTC 2014

Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.

Chemical biology & drug design

Yang H,He X,Zheng Y,Feng W,Xia X,Yu X,Lin Z

Sat Nov 01 00:00:00 UTC 2014

Down-regulation of asparagine synthetase induces cell cycle arrest and inhibits cell proliferation of breast cancer.

Chemical biology & drug design

Yang H,He X,Zheng Y,Feng W,Xia X,Yu X,Lin Z

Sat Nov 01 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: Asparagine synthetase [glutamine-hydrolyzing]; Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase; TS11 cell cycle control protein

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Gene Aliases: ASNS; ASNSD; TS11

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UniProt ID: (Human) P08243

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Entrez Gene ID: (Human) 440

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Function(s)

asparagine synthase (glutamine-hydrolyzing) activity protein binding ATP binding protein homodimerization activity cofactor binding ligase

Process(es)

liver development asparagine biosynthetic process glutamine metabolic process cellular amino acid biosynthetic process response to light stimulus response to mechanical stimulus response to toxic substance response to methotrexate response to follicle-stimulating hormone cellular response to hormone stimulus PERK-mediated unfolded protein response cellular response to glucose starvation negative regulation of apoptotic process response to amino acid positive regulation of mitotic cell cycle L-asparagine biosynthetic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ASNS Polyclonal Antibody

ASNS Antibody (14681-1-AP) in ICC/IF

Product Details

14681-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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