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Invitrogen
ATF3 Polyclonal Antibody
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FIGURE: 1 / 4
ATF3 Antibody (PA5-101089) in ICC/IF
Immunocytochemistry analysis of ATF3 in A549 cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATF3 polyclonal antibody (Product # PA5-101089) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101089
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATF3(Accession P18847), corresponding to amino acid residues K139-S181.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ATF3.
Target Information
ATF3 (Activating transcription factor 3) is a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. It encodes a protein with a calculated molecular mass of 22 kD. ATF3 represses rather than activates transcription from promoters with ATF binding elements. An alternatively spliced form of ATF3 (ATF3 delta Zip) encodes a truncated form ATF3 protein lacking the leucine zipper protein-dimerization motif and does not bind to DNA. In contrast to ATF3, ATF3 delta Zip stimulates transcription presumably by sequestering inhibitory co-factors away from the promoter. It is possible that alternative splicing of the ATF3 gene may be physiologically important in the regulation of target genes. Three transcript variants encoding two different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activating transcription factor 3; ATF 3; cAMP-dependent transcription factor ATF-3; Cyclic AMP-dependent transcription factor ATF-3; Liver regeneration factor 1; LRF-1; Transcription factor LRG-21
Gene Aliases: ATF3; LRF-1; Lrf1; LRFI; LRG-21
UniProt ID: (Human) P18847, (Mouse) Q60765, (Rat) P29596
Entrez Gene ID: (Human) 467, (Mouse) 11910, (Rat) 25389
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcription factor activity, sequence-specific DNA binding
transcription corepressor activity
protein binding
identical protein binding
protein homodimerization activity
transcription regulatory region DNA binding
protein heterodimerization activity
DNA binding
sequence-specific DNA binding
basic leucine zipper transcription factor
negative regulation of transcription from RNA polymerase II promoter
gluconeogenesis
transcription from RNA polymerase II promoter
positive regulation of cell proliferation
cellular response to amino acid starvation
skeletal muscle cell differentiation
PERK-mediated unfolded protein response
positive regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance
negative regulation of ERK1 and ERK2 cascade
positive regulation of TRAIL-activated apoptotic signaling pathway
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
transcription, DNA-templated
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
positive regulation of gene expression
endoplasmic reticulum unfolded protein response
negative regulation of transcription, DNA-templated
cell fate determination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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