Proteintech

ATF4 Polyclonal Antibody

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ATF4 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HepG2 cells using 10835-1-AP (ATF4 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ATF4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10835-1-AP (ATF4 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

ATF4 Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10835-1-AP (ATF4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ATF4 Antibody in Flow Cytometry (Flow) — 1X10^6 HepG2 cells were intracellularly stained with 0.2 µg Anti-Human ATF4 (Product # 10835-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1,000 (red), or 0.2 µg Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

ATF4 Antibody in Immunoprecipitation (IP) — IP result of anti-ATF4 (IP:10835-1-AP, 3ug; Detection:10835-1-AP 1:400) with HEK-293 cells lysate 6000ug.

Product Details

10835-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 85 publications 85 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	View 2 publications 2 publications
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications

Product Specifications
Species Reactivity	Human, Rat
Published species	Human, Mouse, Pig, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATF4 Fusion Protein Ag1279 (1-351 aa encoded by BC022088) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.27 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody is a rabbit polyclonal antibody raised against full length human ATF4 antigen. The antibody recognizes the 38 kDa ATF4 protein and its phosphorylated forms (50 kDa). ATF4 can bind DNA as a homodimer and as a heterodimer. ATF4 is ubiquitinated by SCF(BTRC) in response to mTORC1 signal, followed by proteasomal degradation and leading to down-regulate expression of SIRT4, so the molecular weight of ATF4 may be 70 kDa.

Immunogen sequence: MTEMSFLSS EVLVGDLMSP FDQSGLGAEE SLGLLDDYLE VAKHFKPHGF SSDKAKAGSS EWLAVDGLVS PSNNSKEDAF SGTDWMLEKM DLKEFDLDAL LGIDDLETMP DDLLTTLDDT CDLFAPLVQE TNKQPPQTVN PIGHLPESLT KPDQVAPFTF LQPLPLSPGV LSSTPDHSFS LELGSEVDIT EGDRKPDYTA YVAMIPQCIK EEDTPSDNDS GICMSPESYL GSPQHSPSTR GSPNRSLPSP GVLCGSARPK PYDPPGEKMV AAKVKGEKLD KKLKKMEQNK TAATRYRQKK RAEQEALTGE CKELEKKNEA LKERADSLAK EIQYLKDLIE EVRKARGKKR VP (1-351 aa encoded by BC022088)

Target Information

This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

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Translation of 5' leaders is pervasive in genes resistant to eIF2 repression.

eLife

Andreev DE,O'Connor PB,Fahey C,Kenny EM,Terenin IM,Dmitriev SE,Cormican P,Morris DW,Shatsky IN,Baranov PV

Mon Jan 26 00:00:00 EST 2015

Phosphorylation of the translation initiation factor eIF2α at serine 51 determines the cell fate decisions of Akt in response to oxidative stress.

Cell death & disease

Rajesh K,Krishnamoorthy J,Kazimierczak U,Tenkerian C,Papadakis AI,Wang S,Huang S,Koromilas AE

Thu Jan 15 00:00:00 EST 2015

PERK mediates eIF2α phosphorylation responsible for BACE1 elevation, CREB dysfunction and neurodegeneration in a mouse model of Alzheimer's disease.

Neurobiology of aging

Devi L,Ohno M

Wed Oct 01 00:00:00 EDT 2014

Deletion of the eIF2α Kinase GCN2 fails to rescue the memory decline associated with Alzheimer's disease.

PloS one

Devi L,Ohno M

Tue Aug 05 00:00:00 EDT 2014

HKH40A downregulates GRP78/BiP expression in cancer cells.

Cell death & disease

Kosakowska-Cholody T,Lin J,Srideshikan SM,Scheffer L,Tarasova NI,Acharya JK

Thu May 22 00:00:00 EDT 2014

Ionizing radiation activates PERK/eIF2α/ATF4 signaling via ER stress-independent pathway in human vascular endothelial cells.

International journal of radiation biology

Kim EJ,Lee YJ,Kang S,Lim YB

Tue Apr 01 00:00:00 EDT 2014

Expression of ATF4 and VEGF in chorionic villus tissue in early spontaneous abortion.

European journal of obstetrics, gynecology, and reproductive biology

Chai L,Ling K,He X,Yang R

Tue Oct 01 00:00:00 EDT 2013

Sinulariolide induced hepatocellular carcinoma apoptosis through activation of mitochondrial-related apoptotic and PERK/eIF2α/ATF4/CHOP pathway.

Molecules (Basel, Switzerland)

Chen YJ,Su JH,Tsao CY,Hung CT,Chao HH,Lin JJ,Liao MH,Yang ZY,Huang HH,Tsai FJ,Weng SH,Wu YJ

Thu Aug 22 00:00:00 EDT 2013

Compound C prevents the unfolded protein response during glucose deprivation through a mechanism independent of AMPK and BMP signaling.

PloS one

Saito S,Furuno A,Sakurai J,Park HR,Shin-ya K,Tomida A

Thu Feb 28 00:00:00 EST 2013

Permissive role of miR-663 in induction of VEGF and activation of the ATF4 branch of unfolded protein response in endothelial cells by oxidized phospholipids.

Atherosclerosis

Afonyushkin T,Oskolkova OV,Bochkov VN

Thu Nov 01 00:00:00 EDT 2012

Proteomic profiling of the 11-dehydrosinulariolide-treated oral carcinoma cells Ca9-22: effects on the cell apoptosis through mitochondrial-related and ER stress pathway.

Journal of proteomics

Liu CI,Wang RY,Lin JJ,Su JH,Chiu CC,Chen JC,Chen JY,Wu YJ

Mon Oct 22 00:00:00 EDT 2012

Induction of apoptosis by 11-dehydrosinulariolide via mitochondrial dysregulation and ER stress pathways in human melanoma cells.

Marine drugs

Su TR,Tsai FJ,Lin JJ,Huang HH,Chiu CC,Su JH,Yang YT,Chen JY,Wong BS,Wu YJ

Wed Aug 01 00:00:00 EDT 2012

Role of ER stress response in photodynamic therapy: ROS generated in different subcellular compartments trigger diverse cell death pathways.

PloS one

Moserova I,Kralova J

Mon Jul 09 00:00:00 EDT 2012

Recombinant Lz-8 from Ganoderma lucidum induces endoplasmic reticulum stress-mediated autophagic cell death in SGC-7901 human gastric cancer cells.

Oncology reports

Liang C,Li H,Zhou H,Zhang S,Liu Z,Zhou Q,Sun F

Sun Apr 01 00:00:00 EDT 2012

TDP-43 toxicity is mediated by the unfolded protein response-unrelated induction of C/EBP homologous protein expression.

Journal of neuroscience research

Suzuki H,Matsuoka M

Thu Mar 01 00:00:00 EST 2012

Akt determines cell fate through inhibition of the PERK-eIF2α phosphorylation pathway.

Science signaling

Mounir Z,Krishnamoorthy JL,Wang S,Papadopoulou B,Campbell S,Muller WJ,Hatzoglou M,Koromilas AE

Tue Sep 27 00:00:00 EDT 2011

Doxorubicin bypasses the cytoprotective effects of eIF2α phosphorylation and promotes PKR-mediated cell death.

Cell death and differentiation

Peidis P,Papadakis AI,Muaddi H,Richard S,Koromilas AE

Sat Jan 01 00:00:00 EST 2011

Phosphorylation of eIF2α at serine 51 is an important determinant of cell survival and adaptation to glucose deficiency.

Molecular biology of the cell

Muaddi H,Majumder M,Peidis P,Papadakis AI,Holcik M,Scheuner D,Kaufman RJ,Hatzoglou M,Koromilas AE

Wed Sep 15 00:00:00 EDT 2010

Indoxyl sulfate inhibits proliferation of human proximal tubular cells via endoplasmic reticulum stress.

American journal of physiology. Renal physiology

Kawakami T,Inagi R,Wada T,Tanaka T,Fujita T,Nangaku M

Wed Sep 01 00:00:00 EDT 2010

eIF2alpha Phosphorylation-dependent translation in CA1 pyramidal cells impairs hippocampal memory consolidation without affecting general translation.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Jiang Z,Belforte JE,Lu Y,Yabe Y,Pickel J,Smith CB,Je HS,Lu B,Nakazawa K

Wed Feb 17 00:00:00 EST 2010

Mitochondrial degeneration and not apoptosis is the primary cause of embryonic lethality in ceramide transfer protein mutant mice.

The Journal of cell biology

Wang X,Rao RP,Kosakowska-Cholody T,Masood MA,Southon E,Zhang H,Berthet C,Nagashim K,Veenstra TK,Tessarollo L,Acharya U,Acharya JK

Mon Jan 12 00:00:00 EST 2009

Bioinformatics

Protein Aliases: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor ATF-4; ATF 4; ATF4 protein; atf4 {ECO:0000312|RGD:621863}; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; OTTHUMP00000199130; rATF-4; tax-re; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; TaxREB67

Gene Aliases: ATF4; CREB-2; CREB2; TAXREB67; TXREB

UniProt ID: (Human) P18848, (Rat) Q9ES19

Entrez Gene ID: (Human) 468, (Rat) 79255

Molecular Function: DNA-binding transcription factor

Function(s)

Process(es)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter sequence-specific DNA binding transcription factor activity, RNA polymerase II transcription factor binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding DNA binding transcription factor activity, sequence-specific DNA binding protein binding protein C-terminus binding leucine zipper domain binding sequence-specific DNA binding transcription regulatory region DNA binding protein heterodimerization activity transcription factor binding

ATF4 Polyclonal Antibody

Product Details

10835-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Immunohistochemistry (IHC)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

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Type

Immunogen

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Bioinformatics

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