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Invitrogen
ATF4 Polyclonal Antibody
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FIGURE: 1 / 10
ATF4 Antibody (PA5-27576) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of ATF4 in HeLa cells mock (left) and HeLa cells treated with 2 µg/mL Tunicamycin for 8hrs (right). Cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using ATF4 Polyclonal Antibody (Product # PA5-27576) (Green) at a dilution of 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Product Details
PA5-27576
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human ATF4. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.7 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: HeLa (4 µg/mL tunicamycin treatment for 16 hr).
Predicted reactivity: Mouse (83%), Rat (85%), Pig (89%), Sheep (86%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor ATF-4; ATF 4; ATF4 protein; atf4 {ECO:0000312|RGD:621863}; C/ATF; C/EBP-related ATF; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; OTTHUMP00000199130; rATF-4; tax-re; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; tax-responsive enhancer element-binding protein 67 homolog; TaxREB67; taxREB67 homolog
Gene Aliases: Atf-4; ATF4; C/ATF; CREB-2; CREB2; TAXREB67; TXREB
UniProt ID: (Human) P18848, (Rat) Q9ES19, (Mouse) Q06507
Entrez Gene ID: (Human) 468, (Rat) 79255, (Mouse) 11911
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter sequence-specific DNA binding
transcription factor activity, RNA polymerase II transcription factor binding
transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding
RNA polymerase II transcription factor binding
DNA binding
transcription factor activity, sequence-specific DNA binding
protein binding
protein C-terminus binding
leucine zipper domain binding
sequence-specific DNA binding
transcription regulatory region DNA binding
protein heterodimerization activity
transcription factor binding
DNA-binding transcription factor
gluconeogenesis
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
cellular amino acid metabolic process
gamma-aminobutyric acid signaling pathway
positive regulation of vascular endothelial growth factor production
positive regulation of gene expression
negative regulation of translational initiation in response to stress
circadian regulation of gene expression
cellular response to amino acid starvation
cellular response to UV
response to endoplasmic reticulum stress
positive regulation of transcription from RNA polymerase II promoter in response to stress
positive regulation of transcription from RNA polymerase II promoter in response to oxidative stress
PERK-mediated unfolded protein response
cellular response to glucose starvation
mRNA transcription from RNA polymerase II promoter
positive regulation of apoptotic process
negative regulation of potassium ion transport
positive regulation of neuron apoptotic process
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase I promoter
positive regulation of transcription from RNA polymerase II promoter
positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
negative regulation of oxidative stress-induced neuron death
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
response to manganese-induced endoplasmic reticulum stress
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
rhythmic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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