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Invitrogen
This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:3,000 | - |
Immunocytochemistry (ICC/IF) |
1:100-1:1,000 | - |
ChIP assay (ChIP) |
Assay-dependent | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
Recombinant protein fragment corresponding to a region within amino acids 146 and 333 of Human ATF4 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
1.29 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7, with 20% glycerol |
Contains |
0.025% ProClin 300 |
Storage conditions |
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
RRID |
AB_2545052 |
Recommended positive controls: HeLa (4 µg/mL tunicamycin treatment for 16 hr).
Predicted reactivity: Mouse (83%), Rat (85%), Pig (89%), Sheep (86%), Rhesus Monkey (97%), Chimpanzee (99%), Bovine (90%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
This gene encodes a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain. Two alternative transcripts encoding the same protein have been described. Two pseudogenes are located on the X chromsome at q28 in a region containing a large inverted duplication.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Activating transcription factor 4; activating transcription factor 4 (tax-responsive enhancer element B67); activating transcription factor ATF-4; ATF 4; ATF4 protein; atf4 {ECO:0000312|RGD:621863}; C/ATF; C/EBP-related ATF; cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB 2; CREB-2; Cyclic AMP-dependent transcription factor ATF-4; Cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; OTTHUMP00000199130; rATF-4; tax-re; tax-responsive enhancer element B67; Tax-responsive enhancer element-binding protein 67; tax-responsive enhancer element-binding protein 67 homolog; TaxREB67; taxREB67 homolog
Gene Aliases: Atf-4; ATF4; C/ATF; CREB-2; CREB2; TAXREB67; TXREB
UniProt ID: (Human) P18848, (Rat) Q9ES19, (Mouse) Q06507
Entrez Gene ID: (Human) 468, (Rat) 79255, (Mouse) 11911
Molecular Function:
DNA-binding transcription factor
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