Invitrogen

ATF5 Polyclonal Antibody

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ATF5 Polyclonal Antibody

Product Details

PA5-101276

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:500
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthesized peptide derived from human ATF5(Accession Q9Y2D1), corresponding to amino acid residues A35-P85.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2850715

Product Specific Information

Antibody detects endogenous levels of total ATF5.

Target Information

ATF5 is a protein coding gene. Transcription factor that either stimulates or represses gene transcription through binding of different DNA regulatory elements such as cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), ATF5-specific response element (ARE) (consensus: 5'-C[CT]TCT[CT]CCTT[AT]-3') but also the amino acid response element (AARE), present in many viral and cellular promoters. Critically involved, often in a cell type-dependent manner, in cell survival, proliferation, and differentiation. Its transcriptional activity is enhanced by CCND3 and slightly inhibited by CDK4. Important regulator of the cerebral cortex formation, functions in cerebral cortical neuroprogenitor cells to maintain proliferation and to block differentiation into neurons. Must be down-regulated in order for such cells to exit the cycle and differentiate. Participates in the pathways by which SHH promotes cerebellar granule neuron progenitor cells proliferation. Critical for survival of mature olfactory sensory neurons (OSN), directs expression of OSN-specific genes. May be involved in osteogenic differentiation. Promotes cell proliferation and survival by inducing the expression of EGR1 sinergistically with ELK1. Once acetylated by EP300, binds to ARE sequences on target genes promoters, such as BCL2 and EGR1. Plays an anti-apoptotic role through the transcriptional regulation of BCL2, this function seems to be cell type-dependent. Cooperates with NR1I3/CAR in the transcriptional activation of CYP2B6 in liver. In hepatic cells, represses CRE-dependent transcription and inhibits proliferation by blocking at G2/M phase. May act as a negative regulator of IL1B transduction pathway in liver. Upon IL1B stimulus, cooperates with NLK to activate the transactivation activity of C/EBP subfamily members. Besides its function of transcription factor, acts as a cofactor of CEBPB to activate CEBPA and promote adipocyte differentiation. Regulates centrosome dynamics in a cell-cycle- and centriole-age-dependent manner. Forms 9-foci symmetrical ring scaffold around the mother centriole to control centrosome function and the interaction between centrioles and pericentriolar material.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activating transcription factor 5; Activating transcription factor 5-alpha/beta; activating transcription factor 7; activating transcription factor X; BZIP protein ATF7; cAMP-dependent transcription factor ATF-5; Cyclic AMP-dependent transcription factor ATF-5; FLJ34666; NAP1; NRIF3-associated protein; Transcription factor ATFx; Transcription factor-like protein ODA-10

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Gene Aliases: AFTA; ATF5; Atf7; ATFX; HMFN0395; Nap1; ODA-10

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UniProt ID: (Human) Q9Y2D1, (Rat) Q6P788, (Mouse) O70191

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Entrez Gene ID: (Human) 22809, (Rat) 282840, (Mouse) 107503

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Function(s)
RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription corepressor activity protein binding tubulin binding kinase binding heat shock protein binding sequence-specific DNA binding transcription regulatory region DNA binding DNA binding DNA-binding transcription factor
Process(es)
regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter circadian rhythm negative regulation of cell proliferation post-embryonic development olfactory bulb interneuron development cerebellar granule cell precursor proliferation multicellular organism growth negative regulation of apoptotic process fat cell differentiation negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of centrosome cycle negative regulation of astrocyte differentiation negative regulation of cell cycle G2/M phase transition transcription, DNA-templated regulation of gene expression olfactory bulb interneuron differentiation olfactory lobe development regulation of cell proliferation negative regulation of neurogenesis regulation of cell cycle
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