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Invitrogen
ATF6 Polyclonal Antibody
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FIGURE: 1 / 7
ATF6 Antibody (PA5-20216) in IHC (P)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue using ATF6 Polyclonal Antibody (Product # PA5-20216) at 5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Co... View More
Product Details
PA5-20216
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans,
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 17 amino acid peptide from near the amino terminus of human ATF6.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is MDA-MB-361 cell lysate.
PA5-20216 can be used with blocking peptide PEP-0349.
Target Information
ATF6 is a transcription factor that acts during endoplasmic reticulum stress by activating unfolded protein response target genes. It binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3'). Binding to ERSE requires binding of NF-Y to ERSE. ATF6 could also be involved in activation of transcription by the serum response factor. ATF6 exists as a homodimer and heterodimer with ATF6 beta. The dimer interacts with the nuclear transcription factor Y (NF-Y) trimer through direct binding to NF-Y subunit C (NF-YC). It Interacts also with the transcription factors GTF2I, YY1 and SRF. Under ER stress the cleaved N-terminal cytoplasmic domain translocates into the nucleus. The basic domain of ATF6 functions as a nuclear localization signal and the basic leucine-zipper domain is sufficient for association with the NF-Y trimer and binding to ERSE. During the unfolded protein response an approximately 50 kDa fragment containing the cytoplasmic transcription factor domain is released by proteolysis. The cleavage seems to be performed sequentially by site-1 and site-2 proteases. ATF6 is N-glycosylated, phosphorylated in vitro by MAPK14/P38MAPK and belongs to the bZIP family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Activating transcription factor 6 alpha; Activating transcription factor 6 beta; ATF 6; ATF6 alpha; ATF6-alpha; ATF6-beta; cAMP response element binding protein-related protein; cAMP response element-binding protein-related protein; cAMP responsive element binding protein-like 1; cAMP-dependent transcription factor ATF-6 alpha; cAMP-dependent transcription factor ATF-6 beta; cAMP-responsive element-binding protein-like 1; Creb-related protein; Creb-rp; Cyclic AMP-dependent transcription factor ATF-6 alpha; Cyclic AMP-dependent transcription factor ATF-6 beta; DKFZp686P2194; FLJ21663; Protein G13
Gene Aliases: 9130025P16Rik; 9630036G24; AA617266; AA789574; ACHM7; ATF6; ATF6A; Atf6alpha; ATF6B; ATF6beta; CREB-RP; CREBL1; ESTM49; G13
UniProt ID: (Human) P18850, (Human) Q99941, (Mouse) F6VAN0
Entrez Gene ID: (Human) 22926, (Human) 1388, (Mouse) 226641, (Mouse) 12915
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
transcription coactivator activity
protein binding
ubiquitin protein ligase binding
cAMP response element binding
identical protein binding
protein heterodimerization activity
sequence-specific DNA binding
transcription regulatory region DNA binding
DNA binding
eye development
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
protein folding
response to stress
positive regulation of transcription from RNA polymerase II promoter involved in unfolded protein response
signal transduction
visual perception
endoplasmic reticulum unfolded protein response
ATF6-mediated unfolded protein response
positive regulation of apoptotic process
positive regulation of transcription from RNA polymerase II promoter in response to endoplasmic reticulum stress
regulation of transcription, DNA-templated
response to unfolded protein
positive regulation of transcription from RNA polymerase II promoter
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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