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Invitrogen
ATG10 Polyclonal Antibody
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FIGURE: 1 / 2
ATG10 Antibody (PA5-78593) in WB
ATG10 Polyclonal Antibody detects ATG10 protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 whole cell lysate/extract of human ATG10-transfected 293T cells.12 % SDS-PAGE. ATG10 Polyclonal Antibody (Product # PA5-78593) dilution: 1:5,000.
Product Details
PA5-78593
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human ATG10 Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: human Atg10-transfected 293T
Predicted Reactivity: Mouse (80%), Bovine (84%)
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
ATG10 is involved in autophagy which is a process for the bulk degradation of cytosolic compartments by lysosomes/vacuoles. The formation of autophagosomes involves a dynamic rearrangement of the membrane for which two ubiquitin-like modifications (the conjugation of APG12p and the modification of a soluble form of MAP-LC3 to a membrane-bound form) are essential. APG10 is an E2-like enzyme involved in two ubiquitin-like modifications essential for autophagosome formation, ATG12 -ATG5 conjugation and modification of a soluble form of MAP-LC3 (MAP1LC3A), a homolog of yeast Apg8, to a membrane-bound form. The protein ATG10 is ubiquitously expressed and the human APG10L gene is localized on chromosome 5q14.2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: APG10 autophagy 10-like; APG10-like; ATG10 autophagy related 10 homolog; Autophagy-related protein 10; DKFZp586I0418; FLJ13954; Ubiquitin-like-conjugating enzyme ATG10; unnamed protein product
Gene Aliases: APG10; APG10L; ATG10; PP12616
UniProt ID: (Human) Q9H0Y0
Entrez Gene ID: (Human) 83734
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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