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ATG3 Polyclonal Antibody

ATG3 Antibody in Immunocytochemistry (ICC/IF)

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ATG3 Antibody (11262-2-AP) in ICC/IF

Immunofluorescent analysis of HEK-293 cells using 11262-2-AP (ATG3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

ATG3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

11262-2-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 15 publications 15 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATG3 Fusion Protein Ag1785 (1-314 aa encoded by BC024221)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.13 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MQNVINTVK GKALEVAEYL TPVLKESKFK ETGVITPEEF VAAGDHLVHH CPTWQWATGE ELKVKAYLPT GKQFLVTKNV PCYKRCKQME YSDELEAIIE EDDGDGGWVD TYHNTGITGI TEAVKEITLE NKDNIRLQDC SALCEEEEDE DEGEAADMEE YEESGLLETD EATLDTRKIV EACKAKTDAG GEDAILQTRT YDLYITYDKY YQTPRLWLFG YDEQRQPLTV EHMYEDISQD HVKKTVTIEN HPHLPPPPMC SVHPCRHAEV MKKIIETVAE GGGELGVHMY LLIFLKFVQA VIPTIEYDYT RHFTM (1-314 aa encoded by BC024221)

Target Information

ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Autophagy is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Recombinant humanized collagen type III with high antitumor activity inhibits breast cancer cells autophagy, proliferation, and migration through DDR1.

International journal of biological macromolecules

Liu X,Li H,Wang T,Yang T,Yang X,Guo K,Hu L,Ming J

Sat Jul 15 00:00:00 UTC 2023

Purvalanol A induces apoptosis and reverses cisplatin resistance in ovarian cancer.

Anti-cancer drugs

Zhang X,Hong S,Yang J,Liu J,Wang Y,Peng J,Wang H,Hong L

Sun Jan 01 00:00:00 UTC 2023

HSF4 Transcriptionally Activates Autophagy by Regulating ATG9a During Lens Terminal Differentiation.

Investigative ophthalmology & visual science

Zhang J,Jiang N,Du C,Guo H,Meng R,Hou X,Liu M,Hu Y,Cui X

Thu Jun 01 00:00:00 UTC 2023

EPA and DHA differentially coordinate the crosstalk between host and gut microbiota and block DSS-induced colitis in mice by a reinforced colonic mucus barrier.

Food & function

Fang J,Zhang Z,Cheng Y,Yang H,Zhang H,Xue Z,Lu S,Dong Y,Song C,Zhang X,Zhou Y

Wed Apr 20 00:00:00 UTC 2022

RNA sequence analysis reveals pathways and candidate genes associated with pancreatic acinar cells injury in a mouse pancreatitis model.

Tissue & cell

Chaoqun H,Rong Y,Yunpeng P,Xiaole Z,Wanli G,Chenyuan S,Dongya H,Yi M,Qiang L

Thu Dec 01 00:00:00 UTC 2022

Activation of Autophagy Ameliorates Age-Related Neurogenesis Decline and Neurodysfunction in Adult Mice.

Stem cell reviews and reports

Yang N,Liu X,Niu X,Wang X,Jiang R,Yuan N,Wang J,Zhang C,Lim KL,Lu L

Tue Feb 01 00:00:00 UTC 2022

Analysis of the roles of the Notch1 signalling pathway in modulating deoxynivalenol cytotoxicity.

Ecotoxicology and environmental safety

Xiao Y,Wang J,Wang J,Wang H,Wu S,Bao W

Tue Nov 01 00:00:00 UTC 2022

Toxoplasma gondii CDPK3 Controls the Intracellular Proliferation of Parasites in Macrophages.

Frontiers in immunology

Wu M,An R,Zhou N,Chen Y,Cai H,Yan Q,Wang R,Luo Q,Yu L,Chen L,Du J

Tue Jun 28 00:00:00 UTC 2022

Aquaporin-8 transports hydrogen peroxide to regulate granulosa cell autophagy.

Frontiers in cell and developmental biology

Huang B,Jin L,Zhang L,Cui X,Zhang Z,Lu Y,Yu L,Ma T,Zhang H

Mon Sep 12 00:00:00 UTC 2022

Genome-wide transcriptional profiling and functional analysis reveal miR-330-MAPK15 axis involving in cellular responses to deoxynivalenol exposure.

Chemosphere

Wang H,Zhou Y,Xu C,Cao Y,Xiao Y,Cai D,Uemoto Y,Wu S,Bao W

Fri Jul 01 00:00:00 UTC 2022

Eukaryotic initiation factor 5A2 mediates hypoxia-induced autophagy and cisplatin resistance.

Cell death & disease

Xu G,Chen H,Wu S,Chen J,Zhang S,Shao G,Sun L,Mu Y,Liu K,Pan Q,Li N,An X,Lin S,Chen W

Fri Aug 05 00:00:00 UTC 2022

Exposure to DEHP induces testis toxicity and injury through the ROS/mTOR/NLRP3 signaling pathway in immature rats.

Ecotoxicology and environmental safety

Hong Y,Zhou Y,Shen L,Wei Y,Long C,Fu Y,Wu H,Wang J,Wu Y,Wu S,Wei G

Mon Dec 20 00:00:00 UTC 2021

Resveratrol promotes osteoblastic differentiation in a rat model of postmenopausal osteoporosis by regulating autophagy.

Nutrition & metabolism

Wang W,Zhang LM,Guo C,Han JF

Thu Apr 14 00:00:00 UTC 2022

Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia.

International journal of ophthalmology

Li R,Wang LZ,Du JH,Zhao L,Yao Y

Thu Oct 01 00:00:00 UTC 2020

Autophagy activation and the mechanism of retinal microvascular endothelial cells in hypoxia.

International journal of ophthalmology

Li R,Wang LZ,Du JH,Zhao L,Yao Y

Thu Oct 01 00:00:00 UTC 2020

7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.

Marine drugs

Tsai TC,Lai KH,Su JH,Wu YJ,Sheu JH

Mon Mar 26 00:00:00 UTC 2018

7-Acetylsinumaximol B Induces Apoptosis and Autophagy in Human Gastric Carcinoma Cells through Mitochondria Dysfunction and Activation of the PERK/eIF2α/ATF4/CHOP Signaling Pathway.

Marine drugs

Tsai TC,Lai KH,Su JH,Wu YJ,Sheu JH

Mon Mar 26 00:00:00 UTC 2018

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 UTC 2017

Cytoplasmic fragment of CD147 generated by regulated intramembrane proteolysis contributes to HCC by promoting autophagy.

Cell death & disease

Wu B,Cui J,Yang XM,Liu ZY,Song F,Li L,Jiang JL,Chen ZN

Thu Jul 13 00:00:00 UTC 2017

Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.

Oncotarget

Xiu YL,Sun KX,Chen X,Chen S,Zhao Y,Guo QG,Zong ZH

Tue May 09 00:00:00 UTC 2017

Upregulation of the lncRNA Meg3 induces autophagy to inhibit tumorigenesis and progression of epithelial ovarian carcinoma by regulating activity of ATG3.

Oncotarget

Xiu YL,Sun KX,Chen X,Chen S,Zhao Y,Guo QG,Zong ZH

Tue May 09 00:00:00 UTC 2017

Bioinformatics

Protein Aliases: 2610016C12Rik; APG3-like; ATG3 autophagy related 3 homolog; autophagy-related 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201; Preconditioning-inducible gene 1 protein; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3

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Gene Aliases: 2610016C12Rik; APG3; APG3-LIKE; APG3L; ATG3; Atg3l; PC3-96; PIG-1; Pig1

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UniProt ID: (Human) Q9NT62, (Rat) Q6AZ50, (Mouse) Q9CPX6

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Entrez Gene ID: (Human) 64422, (Rat) 171415, (Mouse) 67841

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Function(s)

protein binding ligase activity Atg8 ligase activity Atg12 transferase activity ubiquitin-like protein transferase activity enzyme binding molecular_function UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity ribosomal S6-glutamic acid ligase activity coenzyme F420-0 gamma-glutamyl ligase activity coenzyme F420-2 alpha-glutamyl ligase activity protein-glycine ligase activity protein-glycine ligase activity, initiating protein-glycine ligase activity, elongating tubulin-glycine ligase activity protein-glutamic acid ligase activity tubulin-glutamic acid ligase activity ubiquitin-protein ligase

Process(es)

autophagosome assembly mitophagy cellular protein modification process protein targeting to membrane macroautophagy protein ubiquitination mitochondrial fragmentation involved in apoptotic process nucleophagy regulation of cilium assembly autophagy protein transport transport

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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ATG3 Polyclonal Antibody

ATG3 Antibody (11262-2-AP) in ICC/IF

Product Details

11262-2-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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