A suggested positive control is mouse kidney tissue lysate.
PA5-20997 can be used with blocking peptide PEP-1111.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
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Protein Aliases: 2610016C12Rik; APG3, APG3-LIKE, APG3L, PC3-96; APG3-like; ATG3 autophagy related 3 homolog; autophagy-related 3; Autophagy-related protein 3; hApg3; Preconditioning-inducible gene 1 protein; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3
Gene Aliases: 2610016C12Rik; APG3; APG3-LIKE; APG3L; ATG3; Atg3l; PC3-96; PIG-1; Pig1
Molecular Function: ligase