Keep as concentrated solution.
Positive Control: HeLa, K562, THP-1, HL-60, Jurkat, Raji, C8D30, NIH-3T3, BCL-1, Raw264.7.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial. For short-term storage (1-2 weeks), product can be stored at 4°C. For long-term storage, aliquot and store product at -20° C or below, avioiding multiple freeze-thaw cycles.
ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. Autophagy is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 2610016C12Rik; 2610016C12Rik antibody; APG3 antibody; APG3-like; APG3-LIKE antibody; APG3L antibody; ATG3 antibody; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3 homolog (S. cerevisiae) antibody; autophagy-related 3; Autophagy-related protein 3; autophagy-related protein 3 antibody; DKFZp564M1178 antibody; FLJ22125 antibody; hApg3; hApg3 antibody; MGC15201 antibody; PC3-96 antibody; Preconditioning-inducible gene 1 protein; Protein PC3-96; Ubiquitin-like-conjugating enzyme ATG3; ubiquitin-like-conjugating enzyme ATG3 antibody
Gene Aliases: 2610016C12Rik; APG3; APG3-LIKE; APG3L; ATG3; Atg3l; PC3-96; PIG-1; Pig1
Molecular Function: ligase