Proteintech

ATG4B Polyclonal Antibody

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Antibody Testing Data (12)

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ATG4B Antibody (15131-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 15131-1-AP) ATG4B antibody at dilution of 1:1,500 incubated at room temperature for 1.5 hours.

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ATG4B Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using (Product # 15131-1-AP) (ATG4B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ATG4B Antibody in Immunoprecipitation (IP) — IP result of anti-ATG4B (IP:15131-1-AP, 4ug; Detection:15131-1-AP 1:500) with mouse skeletal muscle tissue lysate 3600ug.

Product Details

15131-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 17 publications 17 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

ATG4B Fusion Protein Ag7261 (1-393 aa encoded by BC000719)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MDAATLTYD TLRFAEFEDF PETSEPVWIL GRKYSIFTEK DEILSDVASR LWFTYRKNFP AIGGTGPTSD TGWGCMLRCG QMIFAQALVC RHLGRDWRWT QRKRQPDSYF SVLNAFIDRK DSYYSIHQIA QMGVGEGKSI GQWYGPNTVA QVLKKLAVFD TWSSLAVHIA MDNTVVMEEI RRLCRTSVPC AGATAFPADS DRHCNGFPAG AEVTNRPSPW RPLVLLIPLR LGLTDINEAY VETLKHCFMM PQSLGVIGGK PNSAHYFIGY VGEELIYLDP HTTQPAVEPT DGCFIPDESF HCQHPPCRMS IAELDPSIAV GFFCKTEDDF NDWCQQVKKL SLLGGALPMF ELVEQQPSHL ACPDVLNLSL DSSDVERLER FFDSEDEDFE ILSL (1-393 aa encoded by BC000719)

Target Information

Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Inhibition of ATF4-mediated elevation of both autophagy and AKT/mTOR was involved in antitumorigenic activity of curcumin.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Yang J,Bao S,Luo S,Jiang L,Li Q,Kong Y,Cao J

Wed Mar 01 00:00:00 EST 2023

Hydrogen sulfide inhibits human T-cell leukemia virus type-1 (HTLV-1) protein expression via regulation of ATG4B.

Journal of medical virology

Liu H,Sun J,Cheng X,Duan L,Guo S,Zhang Z,Wan J,Wang C,Zhi X,Yuan L,Wang H

Sun Jan 01 00:00:00 EST 2023

m⁶A reader HNRNPA2B1 destabilization of ATG4B regulates autophagic activity, proliferation and olaparib sensitivity in breast cancer.

Experimental cell research

Zheng R,Yu Y,Lv L,Zhang Y,Deng H,Li J,Zhang B

Wed Mar 01 00:00:00 EST 2023

Targeting autophagy peptidase ATG4B with a novel natural product inhibitor Azalomycin F4a for advanced gastric cancer.

Cell death & disease

Zhong L,Yang B,Zhang Z,Wang J,Wang X,Guo Y,Huang W,Wang Q,Cai G,Xia F,Zhou S,Ma S,Nie Y,Lei J,Li M,Liu P,Deng W,Liu Y,Han F,Wang J

Fri Feb 18 00:00:00 EST 2022

Targeting autophagy peptidase ATG4B with a novel natural product inhibitor Azalomycin F4a for advanced gastric cancer.

Cell death & disease

Zhong L,Yang B,Zhang Z,Wang J,Wang X,Guo Y,Huang W,Wang Q,Cai G,Xia F,Zhou S,Ma S,Nie Y,Lei J,Li M,Liu P,Deng W,Liu Y,Han F,Wang J

Fri Feb 18 00:00:00 EST 2022

Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE.

Redox biology

Li W,Luo LX,Zhou QQ,Gong HB,Fu YY,Yan CY,Li E,Sun J,Luo Z,Ding ZJ,Zhang QY,Mu HL,Cao YF,Ouyang SH,Kurihara H,Li YF,Sun WY,Li M,He RR

Fri Aug 05 00:00:00 EDT 2022

Phospholipid peroxidation inhibits autophagy via stimulating the delipidation of oxidized LC3-PE.

Redox biology

Li W,Luo LX,Zhou QQ,Gong HB,Fu YY,Yan CY,Li E,Sun J,Luo Z,Ding ZJ,Zhang QY,Mu HL,Cao YF,Ouyang SH,Kurihara H,Li YF,Sun WY,Li M,He RR

Fri Aug 05 00:00:00 EDT 2022

Curcumin attenuates Cr (VI)-induced cell growth and migration by targeting autophagy-dependent reprogrammed metabolism.

Journal of biochemical and molecular toxicology

Dlamini MB,Bao S,Gao Z,Mei J,Ge H,Jiang L,Geng C,Li Q,Shi X,Liu Y,Cao J

Tue Nov 01 00:00:00 EDT 2022

Discovery and mechanism studies of a novel ATG4B inhibitor Ebselen by drug repurposing and its anti-colorectal cancer effects in mice.

Cell & bioscience

Xie H,Qiang P,Wang Y,Xia F,Liu P,Li M

Wed Dec 21 00:00:00 EST 2022

Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.

Ecotoxicology and environmental safety

Zhou H,Ling H,Li Y,Jiang X,Cheng S,Zubeir GM,Xia Y,Qin X,Zhang J,Zou Z,Chen C

Sat Jan 01 00:00:00 EST 2022

Stratification of diabetic kidney diseases via data-independent acquisition proteomics-based analysis of human kidney tissue specimens.

Frontiers in endocrinology

Huang Q,Fei X,Zhong Z,Zhou J,Gong J,Chen Y,Li Y,Wu X

Tue Dec 06 00:00:00 EST 2022

LncRNA CRNDE Promotes ATG4B-Mediated Autophagy and Alleviates the Sensitivity of Sorafenib in Hepatocellular Carcinoma Cells.

Frontiers in cell and developmental biology

Chen L,Sun L,Dai X,Li T,Yan X,Zhang Y,Xiao H,Shen X,Huang G,Xiang W,Zhang Y,Tan D,Yang S,Nie Y,Huang X,Lian J,He F

Fri Aug 20 00:00:00 EDT 2021

Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway.

Molecular medicine reports

Nie L,Liu M,Chen J,Wu Q,Li Y,Yi J,Zheng X,Zhang J,Chu C,Yang J

Thu Apr 01 00:00:00 EDT 2021

Inhibition of MGMT-mediated autophagy suppression decreases cisplatin chemosensitivity in gastric cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Lei Y,Tang L,Hu J,Wang S,Liu Y,Yang M,Zhang J,Tang B

Fri May 01 00:00:00 EDT 2020

Inhibition of MGMT-mediated autophagy suppression decreases cisplatin chemosensitivity in gastric cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Lei Y,Tang L,Hu J,Wang S,Liu Y,Yang M,Zhang J,Tang B

Fri May 01 00:00:00 EDT 2020

Inhibition of MGMT-mediated autophagy suppression decreases cisplatin chemosensitivity in gastric cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Lei Y,Tang L,Hu J,Wang S,Liu Y,Yang M,Zhang J,Tang B

Fri May 01 00:00:00 EDT 2020

New Anti-Cancer Strategy to Suppress Colorectal Cancer Growth Through Inhibition of ATG4B and Lysosome Function.

Cancers

Fu Y,Gu Q,Luo L,Xu J,Luo Y,Xia F,Han F,Hong L,Yin XM,Huang Z,Li M

Wed Jun 10 00:00:00 EDT 2020

Notoginsenoside R1 Protects Against the Acrylamide-Induced Neurotoxicity via Upregulating Trx-1-Mediated ITGAV Expression: Involvement of Autophagy.

Frontiers in pharmacology

Wang W,Huang L,Thomas ER,Hu Y,Zeng F,Li X

Thu Oct 01 00:00:00 EDT 2020

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Free radical biology & medicine

Li Z,Li Q,Lv W,Jiang L,Geng C,Yao X,Shi X,Liu Y,Cao J

Tue Jan 01 00:00:00 EST 2019

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Free radical biology & medicine

Li Z,Li Q,Lv W,Jiang L,Geng C,Yao X,Shi X,Liu Y,Cao J

Tue Jan 01 00:00:00 EST 2019

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Free radical biology & medicine

Li Z,Li Q,Lv W,Jiang L,Geng C,Yao X,Shi X,Liu Y,Cao J

Tue Jan 01 00:00:00 EST 2019

The interaction of Atg4B and Bcl-2 plays an important role in Cd-induced crosstalk between apoptosis and autophagy through disassociation of Bcl-2-Beclin1 in A549 cells.

Free radical biology & medicine

Li Z,Li Q,Lv W,Jiang L,Geng C,Yao X,Shi X,Liu Y,Cao J

Tue Jan 01 00:00:00 EST 2019

The exosome-like vesicles from osteoarthritic chondrocyte enhanced mature IL-1β production of macrophages and aggravated synovitis in osteoarthritis.

Cell death & disease

Ni Z,Kuang L,Chen H,Xie Y,Zhang B,Ouyang J,Wu J,Zhou S,Chen L,Su N,Tan Q,Luo X,Chen B,Chen S,Yin L,Huang H,Du X,Chen L

Mon Jul 08 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

RUBCN/rubicon and EGFR regulate lysosomal degradative processes in the retinal pigment epithelium (RPE) of the eye.

Autophagy

Muniz-Feliciano L,Doggett TA,Zhou Z,Ferguson TA

Fri Jul 05 00:00:00 EDT 2019

HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.

Cancer letters

Zhang N,Wu Y,Lyu X,Li B,Yan X,Xiong H,Li X,Huang G,Zeng Y,Zhang Y,Lian J,Ni Z,He F

Tue Nov 28 00:00:00 EST 2017

HSF1 upregulates ATG4B expression and enhances epirubicin-induced protective autophagy in hepatocellular carcinoma cells.

Cancer letters

Zhang N,Wu Y,Lyu X,Li B,Yan X,Xiong H,Li X,Huang G,Zeng Y,Zhang Y,Lian J,Ni Z,He F

Tue Nov 28 00:00:00 EST 2017

p62/sequestosome 1 binds to TDP-43 in brains with frontotemporal lobar degeneration with TDP-43 inclusions.

Journal of neuroscience research

Tanji K,Zhang HX,Mori F,Kakita A,Takahashi H,Wakabayashi K

Mon Oct 01 00:00:00 EDT 2012

p62/sequestosome 1 binds to TDP-43 in brains with frontotemporal lobar degeneration with TDP-43 inclusions.

Journal of neuroscience research

Tanji K,Zhang HX,Mori F,Kakita A,Takahashi H,Wakabayashi K

Mon Oct 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: APG4 (ATG4) autophagy-related homolog B; APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagin 1; Autophagin-1; autophagy related 4 homolog B; autophagy related 4B, cysteine peptidase; autophagy-related 4B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; cysteine protease involved in autophagy APG4-B; hAPG4B; HsAPG4B; MmAPG4B

Gene Aliases: 2510009N07Rik; APG4B; ATG4B; Atg4bl; AUTL1; AW048066; KIAA0943

UniProt ID: (Human) Q9Y4P1, (Mouse) Q8BGE6, (Rat) A0A0G2QC33

Entrez Gene ID: (Human) 23192, (Mouse) 66615, (Rat) 316640

Molecular Function: cysteine protease