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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 4 publications 4 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
- | View 1 publication 1 publication |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
ATG4B Fusion Protein Ag7261 (1-393 aa encoded by BC000719) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.54 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: MDAATLTYD TLRFAEFEDF PETSEPVWIL GRKYSIFTEK DEILSDVASR LWFTYRKNFP AIGGTGPTSD TGWGCMLRCG QMIFAQALVC RHLGRDWRWT QRKRQPDSYF SVLNAFIDRK DSYYSIHQIA QMGVGEGKSI GQWYGPNTVA QVLKKLAVFD TWSSLAVHIA MDNTVVMEEI RRLCRTSVPC AGATAFPADS DRHCNGFPAG AEVTNRPSPW RPLVLLIPLR LGLTDINEAY VETLKHCFMM PQSLGVIGGK PNSAHYFIGY VGEELIYLDP HTTQPAVEPT DGCFIPDESF HCQHPPCRMS IAELDPSIAV GFFCKTEDDF NDWCQQVKKL SLLGGALPMF ELVEQQPSHL ACPDVLNLSL DSSDVERLER FFDSEDEDFE ILSL (1-393 aa encoded by BC000719)
Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: APG4 (ATG4) autophagy-related homolog B; APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagin 1; Autophagin-1; autophagy related 4 homolog B; autophagy related 4B, cysteine peptidase; autophagy-related 4B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; cysteine protease involved in autophagy APG4-B; hAPG4B
Gene Aliases: 2510009N07Rik; APG4B; ATG4B; Atg4bl; AUTL1; AW048066; KIAA0943
UniProt ID: (Human) Q9Y4P1, (Mouse) Q8BGE6
Entrez Gene ID: (Human) 23192, (Mouse) 66615, (Rat) 316640
Molecular Function:
cysteine protease
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