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Invitrogen
ATG8 Polyclonal Antibody
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FIGURE: 1 / 1
ATG8 Antibody (PA1-26648) in WB
Western blot of APG8 fusion protein. ATG8 Polyclonal Antibody (Product # PA1-26648) generated by immunization with recombinant yeast APG8 was tested by western blot with other anti-UBL antibodies against E.coli lysates expressing the APG8-GFP fusion protein. All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL. Panel A shows total protein staining using ponceau. Panel B shows specific reaction with APG8 using a 1:4,000 and 1:8,000 dilu... View More
Product Details
PA1-26648
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Yeast
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant yeast Apg8 protein.
Conjugate
Form
Liquid
Concentration
5 mg/mL
Amount
250 µg
Purification
IgG fraction
Storage buffer
PBS, pH 7.2
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-26648 detects a band of approximately 14 kDa. This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.
Target Information
Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Apg8 is required for autophagy (intracellular bulk protein degradation) in yeast. Starved yeast cells take up their own cytoplasm into vacuoles through autophagic bodies. Autophagic bodies form a double-membraned structure called the autophagosome, which subsequently fuses with the vacuole/lysosome. This process similar in mammals. Two sets of genes, APG and AUT, have been identified with this process, and are responsible for two ubiquitin-like systems Apg12 and Apg8, respectively. Apg12 is synthesized in its mature form and seems to have one target, Apg5. Almost all Apg12 molecules are conjugated with Apg5.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG8; AUT7; Autophagy-related protein 8; Autophagy-related ubiquitin-like modifier ATG8; CVT5; Cytoplasm to vacuole targeting protein 5
Gene Aliases: APG8; ATG8; AUT7; CVT5; YBL0732; YBL078C
UniProt ID: (Baker's yeast) P38182
Entrez Gene ID: (Baker's yeast) 852200
mitophagy
ER to Golgi vesicle-mediated transport
autophagy
cellular response to nitrogen starvation
protein transport
macroautophagy
regulation of macroautophagy
protein complex localization
CVT pathway
protein localization to pre-autophagosomal structure
piecemeal microautophagy of nucleus
late nucleophagy
membrane fusion
reticulophagy
protein targeting to vacuole involved in autophagy
lipid droplet formation
regulation of membrane invagination
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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