|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 400-500 of human ATGL.|
|Purification||Antigen affinity chromatography|
|Storage buffer||tris glycine with 150mM NaCl|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Suggested positive control: human adipose tissue lysates and mouse 3T3-L1 cell lysates, antigen standard for PNPLA2 (transient overexpression lysate).
Lipolytic enzymes are required for mobilization of fatty acids from triglyceride stores in adipose tissue. Energy homeostasis is affected by dysfunctional lipolysis and may contribute to the pathogenesis of obesity and insulin resistance. Until recently, hormone-sensitive lipase (HSL) was the only enzyme known to hydrolyze triglycerides in mammalian adipose tissue. It is now thought that a second enzyme, adipose triglyceride lipase (ATGL), catalyzes the initial step in triglyceride hydrolysis. ATGL is highly expressed in adipose tissue of mice and humans. It exhibits high substrate specificity for triacylglycerol and is associated with lipid droplets. Inhibition of ATGL markedly decreases total adipose acyl-hydrolase activity. Thus, ATGL and HSL coordinately catabolize stored triglycerides in adipose tissue of mammals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Adipose triglyceride lipase; Adipose triglyceride lipase antibody; Calcium-independent phospholipase A2; calcium-independent phospholipase A2z (iPL-A2z) antibody; Desnutrin; IPLA2-zeta; mutant patatin-like phospholipase domain containing 2; patatin-like phospholipase domain containing 2; patatin-like phospholipase domain containing protein 2; Patatin-like phospholipase domain-containing protein 2; Pigment epithelium-derived factor; transport secretion protein (TTS) antibody; transport-secretion protein; Transport-secretion protein 2; transport-secretion protein 2.2; triglyceride hydrolase; TTS2; TTS2.2
Gene Aliases: 0610039C21Rik; 1110001C14Rik; ATGL; FP17548; iPLA2zeta; PEDF-R; PNPLA2; TTS-2.2; TTS2
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