Proteintech

ATL3 Polyclonal Antibody

View all (12) ATL3 antibodies

Questions & Answers

Questions & Answers

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ATL3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

ATL3 Antibody in Western Blot (WB) — Mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Western Blot (WB) — SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Western Blot (WB) — HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Western Blot (WB) — Jurkat cells were subjected to SDS PAGE followed by western blot with 16921-1-AP ( ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Western Blot (WB) — HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Western Blot (WB) — HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

ATL3 Antibody in Immunoprecipitation (IP) — IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:1500) with HeLa cells lysate 3200ug.

Product Details

16921-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	1:10-1:100	View 1 publication 1 publication
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	ATL3 Fusion Protein Ag10279 (1-351 aa encoded by BC077727) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.13 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MLSPQRVAA AASRGADDAM ESSKPGPVQV VLVQKDQHSF ELDEKALASI LLQDHIRDLD VVVVSVAGAF RKGKSFILDF MLRYLYSQKE SGHSNWLGDP EEPLTGFSWR GGSDPETTGI QIWSEVFTVE KPGGKKVAVV LMDTQGAFDS QSTVKDCATI FALSTMTSSV QIYNLSQNIQ EDDLQQLQLF TEYGRLAMDE IFQKPFQTLM FLVRDWSFPY EYSYGLQGGM AFLDKRLQVK EHQHEEIQNV RNHIHSCFSD VTCFLLPHPG LQVATSPDFD GKLKDIAGEF KEQLQALIPY VLNPSKLMEK EINGSKVTCR GLLEYFKAYI KIYQGEDLPH PKSMLQATAE AN (1-351 aa encoded by BC077727)

Target Information

ATL3 is GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. ATL3 is function in endoplasmic reticulum tubular network biogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 EDT 2018

DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.

Cell research

Zheng P,Chen Q,Tian X,Qian N,Chai P,Liu B,Hu J,Blackstone C,Zhu D,Teng J,Chen J

Wed Aug 01 00:00:00 EDT 2018

Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology.

eLife

Pawar S,Ungricht R,Tiefenboeck P,Leroux JC,Kutay U

Mon Aug 14 00:00:00 EDT 2017

Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology.

eLife

Pawar S,Ungricht R,Tiefenboeck P,Leroux JC,Kutay U

Mon Aug 14 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: atlastin 3; Atlastin-3

Gene Aliases: 4633402C03Rik; 5730596K20Rik; AI465397; ATL3; AW228836; HSN1F; RGD1309871

UniProt ID: (Human) Q6DD88, (Rat) Q0ZHH6, (Mouse) Q91YH5

Entrez Gene ID: (Human) 25923, (Rat) 309187, (Mouse) 109168

Molecular Function: heterotrimeric G-protein

ER to Golgi vesicle-mediated transport endoplasmic reticulum organization Golgi organization protein homooligomerization metabolic process

Process(es)

GTPase activity GTP binding identical protein binding nucleotide binding hydrolase activity