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          • Primary Antibodies ›
          • ATM Antibodies

          Invitrogen

          ATM Polyclonal Antibody

          Advanced Verification
          View all (80) ATM antibodies

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          Cite ATM Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          • Advanced Verification (1)
          ATM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ATM Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 4

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          ATM Antibody (PA5-85299) in ICC/IF

          Immunocytochemistry analysis of ATM in paraformaldehyde-fixed HeLa. Samples were incubated with ATM polyclonal antibody (Product # PA5-85299) using a dilution of 1:500. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          ATM Antibody in Immunocytochemistry (ICC/IF)
          ATM Antibody in Western Blot (WB)
          ATM Antibody in Western Blot (WB)
          ATM Antibody

          Product Details

          PA5-85299

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:3,000
          -

          Immunohistochemistry (Paraffin) (IHC (P))

          Assay-dependent
          -

          Immunocytochemistry (ICC/IF)

          1:100-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant protein encompassing a sequence within the center region of human ATM. The exact sequence is proprietary.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.93 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          0.1M tris glycine, pH 7, with 20% glycerol

          Contains

          0.01% thimerosal

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2792442

          Product Specific Information

          Keep as concentrated solution.

          Predicted reactivity: Dog (84%), Pig (81%), Bovine (82%).

          Positive Control: HeLa, HeLa NE.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          Target Information

          Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM's C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

          ⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: A-T; A-T mutated; AT mutated; AT mutated protein; Ataxia t; Ataxia telangiectasia mutated; DKFZp781A0353; EC 2.7.1.37; MGC74674; mutated in ataxia telangiectasia; Serine-protein kinase ATM; serine/threonine kinase ATM; TEL1, telomere maintenance 1, homolog; unnamed protein product

          View more View less

          Gene Aliases: AT1; ATA; ATC; ATD; ATDC; ATE; ATM; TEL1; TELO1

          View more View less

          UniProt ID: (Human) Q13315

          View more View less

          Entrez Gene ID: (Human) 472

          View more View less

          Function(s)
          nucleotide binding DNA binding protein kinase activity protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding kinase activity 1-phosphatidylinositol-3-kinase activity transferase activity histone H2AXS139 kinase activity identical protein binding macromolecular complex binding protein serine kinase activity
          Process(es)
          autophagosome assembly DNA damage checkpoint pexophagy telomere maintenance double-strand break repair via homologous recombination DNA double-strand break processing ovarian follicle development somitogenesis pre-B cell allelic exclusion immune system process DNA repair double-strand break repair double-strand break repair via nonhomologous end joining chromatin remodeling protein phosphorylation protein monoubiquitination cellular response to DNA damage stimulus response to oxidative stress mitotic spindle assembly checkpoint mitotic G2 DNA damage checkpoint meiosis I reciprocal meiotic recombination male meiosis female meiotic division signal transduction female gamete generation brain development heart development determination of adult lifespan female gonad development intrinsic apoptotic signaling pathway in response to DNA damage post-embryonic development response to ionizing radiation regulation of gene expression regulation of autophagy positive regulation of macromolecule biosynthetic process positive regulation of gene expression DNA damage response, signal transduction by p53 class mediator positive regulation of cell migration negative regulation of B cell proliferation SCF-dependent proteasomal ubiquitin-dependent protein catabolic process regulation of telomere maintenance via telomerase positive regulation of telomere maintenance via telomerase V(D)J recombination cellular response to stress cellular response to reactive oxygen species multicellular organism growth phosphatidylinositol-3-phosphate biosynthetic process peptidyl-serine autophosphorylation lipoprotein catabolic process response to starvation signal transduction in response to DNA damage regulation of apoptotic process positive regulation of apoptotic process proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of neuron apoptotic process meiotic telomere clustering positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter protein autophosphorylation thymus development oocyte development protein stabilization neuron apoptotic process regulation of cell cycle chromosome organization involved in meiotic cell cycle protein K63-linked ubiquitination histone mRNA catabolic process cellular response to retinoic acid cellular response to gamma radiation cellular response to X-ray cellular response to nitrosative stress regulation of cellular response to stress cellular senescence replicative senescence establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere DNA strand resection involved in replication fork processing regulation of cellular response to heat cellular response to oxygen-containing compound regulation of signal transduction by p53 class mediator negative regulation of signal transduction by p53 class mediator negative regulation of TORC1 signaling negative regulation of telomere capping positive regulation of telomere maintenance via telomere lengthening positive regulation of telomerase catalytic core complex assembly positive regulation of double-strand break repair via homologous recombination protein localization to site of double-strand break negative regulation of double-strand break repair via homologous recombination positive regulation of double-strand break repair regulation of autophagosome assembly positive regulation of autophagosome assembly
          It has to be done as per old AB suggested Products section.

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