Invitrogen

ATP Citrate Lyase Polyclonal Antibody

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This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

2 Published Figures

4 References

View all (34) ATP Citrate Lyase antibodies

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ATP Citrate Lyase Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of ATP Citrate Lyase was performed using 70% confluent log phase A549 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29495) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415). Panel d represents the merged image showing cytoplasmic localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ATP Citrate Lyase Antibody in Western Blot (WB) — Knockdown of ATP Citrate Lyase was achieved by transfecting A549 cells with ATP Citrate Lyase specific siRNAs (Silencer® select Product # s916). Western blot analysis (Fig. a) was performed using whole cell extracts from the ATP Citrate Lyase knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29495, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to ATP Citrate Lyase.

ATP Citrate Lyase Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. A549 cells were transfected with ATP Citrate Lyase siRNA and reduction of signal was observed in Western Blot using ATP Citrate Lyase Polyclonal Antibody (Product # PA5-29495). {KD}

Product Details

PA5-29495

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 743 and 1038 of Human ATP citrate lyase View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	0.1M tris glycine, pH 7, with 10% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2546971

Product Specific Information

Recommended positive controls: HeLa, Raji, mouse brain, PC-12, Rat-2.

Predicted reactivity: Mouse (97%), Rat (97%), Xenopus laevis (91%), Pig (98%), Chicken (95%), Sheep (99%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Two transcript variants encoding distinct isoforms have been identified for this gene.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

BCAA Supplementation in Mice with Diet-induced Obesity Alters the Metabolome Without Impairing Glucose Homeostasis.

Endocrinology

Lee J,Vijayakumar A,White PJ,Xu Y,Ilkayeva O,Lynch CJ,Newgard CB,Kahn BB

PA5-29495 was used in Western Blotting to highlight major differences in the effects of BCAAs on glucose homeostasis in diet-induced obese mice versus data reported in obese rats and in humans.

Thu Jul 01 00:00:00 EDT 2021

Dysregulated transmethylation leading to hepatocellular carcinoma compromises redox homeostasis and glucose formation.

Molecular metabolism

Hughey CC,James FD,Wang Z,Goelzer M,Wasserman DH

PA5-29495 was used in Western Blotting to test that nutrient flux pivots from glucose production to pathways that incorporate and metabolise methyl groups in GNMT-null mice with hepatocellular carcinoma.

Wed May 01 00:00:00 EDT 2019

Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates.

The Journal of biological chemistry

Hughey CC,Trefts E,Bracy DP,James FD,Donahue EP,Wasserman DH

Published figure using ATP Citrate Lyase polyclonal antibody (Product # PA5-29495) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

Glycine N-methyltransferase deletion in mice diverts carbon flux from gluconeogenesis to pathways that utilize excess methionine cycle intermediates.

The Journal of biological chemistry

Hughey CC,Trefts E,Bracy DP,James FD,Donahue EP,Wasserman DH

Published figure using ATP Citrate Lyase polyclonal antibody (Product # PA5-29495) in Western Blot

Fri Jul 27 00:00:00 EDT 2018

Extended Multiplexing of Tandem Mass Tags (TMT) Labeling Reveals Age and High Fat Diet Specific Proteome Changes in Mouse Epididymal Adipose Tissue.

Molecular & cellular proteomics : MCP

Plubell DL,Wilmarth PA,Zhao Y,Fenton AM,Minnier J,Reddy AP,Klimek J,Yang X,David LL,Pamir N

PA5-29495 was used in Western Blotting to identify that both age-related and high fat diet specific proteomic signatures highlight a pronounced involvement of arginine metabolism in response to advancing age, and branched chain amino acid metabolism in early response to high fat feeding.