Invitrogen

ATP Synthase beta Polyclonal Antibody

Product Image Gallery

ATP Synthase beta Antibody in Immunofluorescence (IF) — Immunofluorescence analysis of NIH/3T3 cells using ATP5B Polyclonal antibody (Product # PA5-96419) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ATP Synthase beta Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP5B Polyclonal antibody (Product # PA5-96419) at a dilution of 1:100 (40x lens).

ATP Synthase beta Antibody in Western Blot (WB) — Western blot analysis of extracts of various cell lines, using ATP5B Polyclonal antibody (Product # PA5-96419) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 3s.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1:50 - 1:200

Immunofluorescence (IF)

1:50 - 1:200

Immunohistochemistry (Paraffin) (IHC (P))

1:50 - 1:200

Western Blot (WB)

1:500 - 1:2000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B.

Conjugate

Unconjugated

Form

Liquid

Concentration

4.54 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Immunogen sequence: YSVFAGVGER TREGNDLYHE MIESGVINLK DATSKVALVY GQMNEPPGAR ARVALTGLTV AEYFRDQEGQ DVLLFIDNIF RFTQAGSEVS ALLGRIPSAV GYQPTLATDM GTMQERITTT KKGSITSVQA IYVPADDLTD PAPATTFAHL DATTVLSRAI AELGIYPAVD PLDSTSRIMD PNIVGSEHYD VARGVQKILQ DYKSLQDIIA ILGMDELSEE DKLTVSRARK IQRFLSQPFQ VAEVFTGHMG KLVPLKETIK GFQQILAGEY DHLPEQAFYM VGPIEEAVAK ADKLAEEHSS; Positive Samples: SW620, BT-474, BxPC-3, HepG2, 293T, Mouse kidney, Mouse heart, Mouse skeletal muscle, Mouse brain; Cellular Location: Mitochondrion, Mitochondrion inner membrane

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947

Molecular Function: ATP synthase DNA binding protein anion channel cation transporter hydrolase ion channel ligand-gated ion channel nucleic acid binding receptor transporter