Invitrogen

ATP Synthase beta Polyclonal Antibody

Product Image Gallery

ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of ATP Synthase beta was performed in NIH/3T3 cells using ATP Synthase beta Polyclonal Antibody (Product # PA5-96419) at a dilution of 1:100. Blue: DAPI for nuclear staining.

ATP Synthase beta Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of ATP Synthase beta in paraffin-embedded human gastric cancer using ATP Synthase beta Polyclonal Antibody (Product # PA5-96419) at a dilution of 1:100.

ATP Synthase beta Antibody in Western Blot (WB) — Western Blot analysis of ATP Synthase beta in extracts of various cell lines using ATP Synthase beta Polyclonal Antibody (Product # PA5-96419) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Product Details

PA5-96419

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50 - 1:200	-
Immunocytochemistry (ICC/IF)	1:50 - 1:200	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP5B. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	4.54 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808221

Product Specific Information

Immunogen sequence: YSVFAGVGER TREGNDLYHE MIESGVINLK DATSKVALVY GQMNEPPGAR ARVALTGLTV AEYFRDQEGQ DVLLFIDNIF RFTQAGSEVS ALLGRIPSAV GYQPTLATDM GTMQERITTT KKGSITSVQA IYVPADDLTD PAPATTFAHL DATTVLSRAI AELGIYPAVD PLDSTSRIMD PNIVGSEHYD VARGVQKILQ DYKSLQDIIA ILGMDELSEE DKLTVSRARK IQRFLSQPFQ VAEVFTGHMG KLVPLKETIK GFQQILAGEY DHLPEQAFYM VGPIEEAVAK ADKLAEEHSS; Positive Samples: SW620, BT-474, BxPC-3, HepG2, 293T, Mouse kidney, Mouse heart, Mouse skeletal muscle, Mouse brain; Cellular Location: Mitochondrion, Mitochondrion inner membrane

Target Information

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this product

Bioinformatics

Protein Aliases: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271

UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480

Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947

Molecular Function: ATP synthase