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ATP Synthase beta Antibody (PA5-96419) in WB
Western blot analysis of ATP Synthase beta in various lysates. Samples were incubated with ATP Synthase beta Polyclonal antibody (Product # PA5-96419) using a dilution of 1:400, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-96419
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A recombinant fusion protein containing a sequence corresponding to amino acids 230-529 of human ATP Synthase beta.
Conjugate
Unconjugated
Form
Liquid
Concentration
4.54 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.05% ProClin 300
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2808221
Product Specific Information
Immunogen sequence: YSVFAGVGER TREGNDLYHE MIESGVINLK DATSKVALVY GQMNEPPGAR ARVALTGLTV AEYFRDQEGQ DVLLFIDNIF RFTQAGSEVS ALLGRIPSAV GYQPTLATDM GTMQERITTT KKGSITSVQA IYVPADDLTD PAPATTFAHL DATTVLSRAI AELGIYPAVD PLDSTSRIMD PNIVGSEHYD VARGVQKILQ DYKSLQDIIA ILGMDELSEE DKLTVSRARK IQRFLSQPFQ VAEVFTGHMG KLVPLKETIK GFQQILAGEY DHLPEQAFYM VGPIEEAVAK ADKLAEEHSS; Positive Samples: SW620, BT-474, BxPC-3, HepG2, 293T, Mouse kidney, Mouse heart, Mouse skeletal muscle, Mouse brain; Cellular Location: Mitochondrion, Mitochondrion inner membrane
Target Information
ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; epididymis secretory protein Li 271; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit
Gene Aliases: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271
UniProt ID: (Human) P06576, (Rat) P10719, (Mouse) P56480
Entrez Gene ID: (Human) 506, (Rat) 171374, (Mouse) 11947
Molecular Function:
ATP synthase
angiogenesis
osteoblast differentiation
generation of precursor metabolites and energy
lipid metabolic process
ATP biosynthetic process
negative regulation of cell adhesion involved in substrate-bound cell migration
mitochondrion organization
ATP hydrolysis coupled proton transport
proton transport
mitochondrial ATP synthesis coupled proton transport
regulation of intracellular pH
receptor-mediated endocytosis
ATP synthesis coupled proton transport
ATP metabolic process
transport
ion transport
hydrogen ion transmembrane transport
transporter activity
protein binding
ATP binding
ATPase activity
transmembrane transporter activity
MHC class I protein binding
proton-transporting ATP synthase activity, rotational mechanism
proton-transporting ATPase activity, rotational mechanism
calcium ion binding
lipoprotein particle receptor activity
nucleotide binding
hydrolase activity
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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