Invitrogen

ATP1B2 Polyclonal Antibody

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ATP1B2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of formalin-fixed, paraffin-embedded human hepatocarcinoma tissue using an ATP1B2 polyclonal antibody (Product # PA5-26279), followed by HRP-conjugated secondary antibody and DAB staining.

ATP1B2 Antibody in Western Blot (WB) — Western blot analysis using an ATP1B2 polyclonal antibody (Product # PA5-26279) in K562 cell lysates (35 µg per lane).

ATP1B2 Antibody in Flow Cytometry (Flow) — Flow cytometry analysis of K562 cells using an ATP1B2 polyclonal antibody (Product # PA5-26279) (bottom) compared to a negative control cell (top) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody

ATP1B2 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Fig. 3 Reduced L1 expression leads to elevated AMOG expression. a-c Western blot analysis of L1 and AMOG expression in U-87 MG ( a ), U251 ( b ), and SHG44 ( c ) cells after treatment with L1 siRNA at 5 nM. Mean values +- SEM from 4 independent experiments are presented. (* p < 0.05, ** p < 0.01, *** p < 0.001 vs. Control siRNA, independent Student's t -test). d-f Representative images of double immunofluorescence staining of L1 and AMOG in response to L1 siRNA at 5 nM in U-87 MG ( d ), U251 ( e ) and SHG44 ( f ) cells. Scale bars = 50 mum

ATP1B2 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC) — Additional file 2: Figure S2. Expression of AMOG in relation to L1. Representative images of double immunofluorescence staining of L1 and AMOG after treatment with L1 siRNA at 10 and 20 nM in U-87 MG ( a ), U251 ( b ) and SHG44 ( c ) cells. Scale bars = 50 mum. (TIF 3736 kb)

ATP1B2 Antibody in Immunohistochemistry, Peptide array (IHC, ARRAY) — Fig. 1 AMOG expression as assayed in a normal human brain and glioma tissue microarray. a Immunostaining of AMOG in the microarray containing both tumor-adjacent normal human brain and glioma tissues graded from I to IV. b Gray-scale image showing AMOG expression in all samples. c Samples from the tissue microarray were pooled in accordance with the WHO grading system. d Data were collected from all the samples arrayed in the slides for quantification. Values are expressed as means +- SEM. * p < 0.05, compared with normal brain tissues; # p < 0.05, compared with grade I glioma tissues; one-way ANOVA with Tukey's post-hoc test. Samples of normal brain tissue ( n = 18), pilocytic astrocytoma (WHO grade I, n = 23), WHO grade I-II ( n = 19), diffuse astrocytoma (WHO grade II, n = 85), anaplastic astrocytoma (WHO grade III, n = 27), and glioblastoma (WHO grade IV, n = 31). e Representative AEC-based AMOG immunostained images in normal human brain tissues and different grades of human glioma tissues. Photographs were taken with a digital microscope. Scale bars, upper panels 500 mum, middle panels 200 mum, and lower panels 20 mum

ATP1B2 Antibody in Western Blot, Flow Cytometry (WB, Flow) — Fig 6 Retinoschisin binding to ATP1B2_T240A and ATP1B2_T240S. HEK293 cells were transfected with expression constructs for ATP1A3 in combination with expression constructs for ATP1B2_T240A and ATP1B2_T240S. (A) 48 h after transfection, cells were subjected to recombinant retinoschisin for 1 h, followed by intensive washing. Cells transfected with expression constructs for only ATP1B2 served as a negative control, cells transfected with expression constructs for ATP1A3 and normal ATP1B2 served as a positive control in the retinoschisin binding assay [ 13 ]. Heterologous protein expression as well as retinoschisin binding was investigated by Western blot analyses with antibodies against retinoschisin, ATP1A3, and ATP1B2. The ACTB staining served as loading control. (B) 48 h after transfection, cells were subjected to FACS analyses applying an anti-ATP1B2 antibody, representative histograms are given in this figure. Light grey: histogram of untransfected HEK293 cells depicting unspecific background signals. Dark grey: histogram of transfected HEK293 cells.

Product Details

PA5-26279

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:100	-
Flow Cytometry (Flow)	1:10-1:50	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 3 publications 3 publications
Peptide Array (Array)	-	View 1 publication 1 publication
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human, Mouse
Host/Isotype	Rabbit / Ig
Class	Polyclonal
Type	Antibody
Immunogen	KLH conjugated synthetic peptide between 115-141 amino acids from the central region of human ATP1B2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A, Antigen affinity chromatography
Storage buffer	PBS
Contains	0.09% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2543779

Product Specific Information

This antibody is predicted to react with bovine, mouse, rat and rabbit based on sequence homology.

Target Information

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-2 subunit is not known. Mediates cell adhesion of neurons and astrocytes, and promotes neurite outgrowth.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Identification of the retinoschisin-binding site on the retinal Na/K-ATPase.

PloS one

Plössl K,Straub K,Schmid V,Strunz F,Wild J,Merkl R,Weber BHF,Friedrich U

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Identification of the retinoschisin-binding site on the retinal Na/K-ATPase.

PloS one

Plössl K,Straub K,Schmid V,Strunz F,Wild J,Merkl R,Weber BHF,Friedrich U

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression.

BMC cancer

Jiang Q,Xie Q,Hu C,Yang Z,Huang P,Shen H,Schachner M,Zhao W

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression.

BMC cancer

Jiang Q,Xie Q,Hu C,Yang Z,Huang P,Shen H,Schachner M,Zhao W

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression.

BMC cancer

Jiang Q,Xie Q,Hu C,Yang Z,Huang P,Shen H,Schachner M,Zhao W

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

Glioma malignancy is linked to interdependent and inverse AMOG and L1 adhesion molecule expression.

BMC cancer

Jiang Q,Xie Q,Hu C,Yang Z,Huang P,Shen H,Schachner M,Zhao W

Published figure using ATP1B2 polyclonal antibody (Product # PA5-26279) in Immunofluorescence

Thu Sep 12 00:00:00 EDT 2019

A SINE Insertion in ATP1B2 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA2).

G3 (Bethesda, Md.)

Mauri N,Kleiter M,Dietschi E,Leschnik M,Högler S,Wiedmer M,Dietrich J,Henke D,Steffen F,Schuller S,Gurtner C,Stokar-Regenscheit N,O'Toole D,Bilzer T,Herden C,Oevermann A,Jagannathan V,Leeb T

PA5-26279 was used in Immunohistochemistry to identify ∼10.6 Mb critical interval on chromosome 5 in a Malinois family with four puppies affected by cerebellar dysfunction.

Mon Aug 07 00:00:00 EDT 2017

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Molecular biology of the cell

Plössl K,Royer M,Bernklau S,Tavraz NN,Friedrich T,Wild J,Weber BHF,Friedrich U

PA5-26279 was used in Immunohistochemistry to study the influence of retinoschisin on the functionality of the Na/K-ATPase.

Tue Aug 01 00:00:00 EDT 2017

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Molecular biology of the cell

Plössl K,Royer M,Bernklau S,Tavraz NN,Friedrich T,Wild J,Weber BHF,Friedrich U

PA5-26279 was used in Immunohistochemistry to study the influence of retinoschisin on the functionality of the Na/K-ATPase.

Tue Aug 01 00:00:00 EDT 2017

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Molecular biology of the cell

Plössl K,Royer M,Bernklau S,Tavraz NN,Friedrich T,Wild J,Weber BHF,Friedrich U

PA5-26279 was used in Immunohistochemistry to study the influence of retinoschisin on the functionality of the Na/K-ATPase.

Tue Aug 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Adhesion molecule in glia; adhesion molecule on glia; AMOG; ATPase, Na+/K+ transporting, beta 2 polypeptide; Na, K-ATPase beta-2 polypeptide; Sodium Potassium ATPase; sodium pump subunit beta-2; sodium-potassium ATPase subunit beta 2 (non-catalytic); sodium/potassium-dependent ATPase beta-2 subunit; Sodium/potassium-dependent ATPase subunit beta-2; sodium/potassium-transporting ATPase beta-2 chain; Sodium/potassium-transporting ATPase subunit beta-2

Gene Aliases: AMOG; ATP1B2

UniProt ID: (Human) P14415

Entrez Gene ID: (Human) 482

Molecular Function: primary active transporter

Function(s)

transport cellular sodium ion homeostasis cell adhesion potassium ion import establishment or maintenance of transmembrane electrochemical gradient cellular potassium ion homeostasis positive regulation of ATPase activity ion transmembrane transport sodium ion export from cell protein stabilization leukocyte migration membrane repolarization cell communication by electrical coupling involved in cardiac conduction ATP hydrolysis coupled transmembrane transport positive regulation of potassium ion transmembrane transporter activity positive regulation of sodium ion export from cell positive regulation of potassium ion import regulation of cardiac conduction

Process(es)

ATPase activator activity sodium:potassium-exchanging ATPase activity protein binding ATPase binding

ATP1B2 Polyclonal Antibody

Product Details

PA5-26279

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Flow Cytometry (Flow)

Immunohistochemistry (IHC)

Peptide Array (Array)

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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