Invitrogen

ATP2A1 Polyclonal Antibody

View all (22) SERCA1 ATPase antibodies

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Protocols

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ATP2A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ATP2A1 in paraffin-embedded human fetal skeletal muscle tissue. Samples were incubated with ATP2A1 polyclonal antibody (Product # PA5-109288), and followed by hematoxylin.

ATP2A1 Antibody in Western Blot (WB) — Western blot analysis of ATP2A1 in Lane 1: Mouse skeletal muscle tissue, Lane 2: Human skeletal muscle tissue. Samples were incubated with ATP2A1 polyclonal antibody (Product # PA5-109288), at a dilution of 1:500.

ATP2A1 Antibody in Flow Cytometry (Flow) — Flow cytometry of ATP2A1 in 293T cells (fuchsia) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with ATP2A1 polyclonal antibody (Product # PA5-109288) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.

Product Details

PA5-109288

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetis peptide within mouse ATP2A1 aa 1-50 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2854699

Product Specific Information

Positive Control: Human skeletal muscle tissue, mouse skeletal muscle tissue, rat skeletal muscle tissue, 293T.

Target Information

ATP dependent calcium pumps are responsible, in part, for the maintenance of low cytoplasmic free calcium concentrations. The ATP pumps that reside in intracellular organelles are encoded by a family of structurally related enzymes, termed the sarcoplasmic or endoplasmic reticulum calcium (SERCA) ATPases. The SERCA1 gene is exclusively expressed in type II (fast) skeletal muscle. The SERCA2 gene is subject to tissue dependent processing which is responsible for the generation of SERCA2a muscle-specific form expressed in type I (slow) skeletal, cardiac and smooth muscle and the SERCA2b isoform expressed in all cell types. The SERCA3 gene is not as well characterized and is found in non-muscle cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA; SERCA ATPase; SERCA1; SR Ca(2+)-ATPase 1

Gene Aliases: ATP2A; ATP2A1; SERCA1

UniProt ID: (Human) O14983, (Mouse) Q8R429, (Rat) Q64578

Entrez Gene ID: (Human) 487, (Mouse) 11937, (Rat) 116601

Molecular Function: primary active transporter