A suggested positive control is NIH-3T3 cell lysate.
PA5-21127 can be used with blocking peptide PEP-1241.
ATP2C2, also known as secretory pathway Ca2+/Mn2+-ATPase (SPCA) 2, belongs to the family of P-type cation transport ATPases. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium from the cytosol to the Golgi lumen. Defects in the related gene ATP2C1 cause Hailey-Hailey disease, for which ATP2C2 does not compensate, suggesting that ATP2C2 plays other physiological roles. Unlike ATP2C1, ATP2C2 has a much more restricted expression pattern and displays a higher maximal turnover rate for overall Ca2+-ATPase reaction and a lower apparent affinity for cytosolic Ca2+ activation of phosphorylation. Overexpression of ATP2C2 in mammary tumors result a Ca2+ influx via the store-operated Ca2+ channel ORAI1 and independent of the STIM1 and STIM2 sensors.
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Protein Aliases: ATPase 2C2; ATPase, Ca++ transporting, type 2C, member 2; Calcium-transporting ATPase type 2C member 2; Secretory pathway Ca(2+)-ATPase 2; secretory pathway Ca2+-ATPase; secretory pathway calcium ATPase 2
Gene Aliases: 1810010G06Rik; ATP2C2; KIAA0703; mKIAA0703; SPCA2