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Invitrogen
ATP4A Polyclonal Antibody
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FIGURE: 1 / 3
ATP4A Antibody (PA5-143987) in IHC (P)
Immunohistochemistry analysis of ATP4A in paraffin-embedded section of rat stomach tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. Samples were incubated with ATP4A Polyclonal antibody (Product # PA5-143987) using a dilution of 2 μg/mL overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG wa... View More
Product Details
PA5-143987
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human ATP4A (AELEQKYQTSATKGLSASLAAELLLRD).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: rat testis tissue, rat stomach tissue, mouse testis tissue, mouse stomach tissue. IHC: rat stomach tissue. Flow: Daudi cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
The ATP4A gene encodes the alpha subunit of the gastric H+/K+ ATPase, an enzyme crucial for gastric acid secretion in the stomach. This proton pump resides in the parietal cells of the gastric mucosa and is responsible for exchanging potassium ions (K+) for hydrogen ions (H+), effectively acidifying the stomach contents. ATP4A plays a significant role in maintaining the acidic environment necessary for digestion and defense against pathogens. The ATPase's activity is tightly regulated by various factors, including hormones like gastrin and histamine, to ensure optimal stomach function. Mutations in ATP4A can lead to disorders related to gastric acidity, such as hypochlorhydria (low stomach acid), which can impact digestion and absorption of nutrients. Understanding ATP4A's function and its regulatory mechanisms is essential for developing treatments for conditions like peptic ulcer disease and gastroesophageal reflux disease, where gastric acid secretion is disrupted.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase H+/K+ transporting alpha subunit; ATPase, H+/K+ exchanging, alpha polypeptide; ATPase, H+/K+ transporting, alpha polypeptide; epididymis secretory sperm binding protein; Gastric H(+)/K(+) ATPase subunit alpha; gastric H+/K+ ATPase alpha subunit; gastric H,K-ATPase catalytic subunit; gastric hydrogen-potassium ATPase; H+/K+-ATPase alpha; H+K+-transporting alpha 1; hydrogen/potassium-exchanging ATPase 4A; Potassium-transporting ATPase alpha chain 1; Proton pump
Gene Aliases: ATP4A; ATP6A; Hka; Hkatpc
UniProt ID: (Human) P20648, (Mouse) Q64436
Entrez Gene ID: (Human) 495, (Rat) 24216, (Mouse) 11944
nucleotide binding
magnesium ion binding
sodium:potassium-exchanging ATPase activity
ATP binding
potassium-transporting ATPase activity
hydrogen:potassium-exchanging ATPase activity
ATPase activity
potassium ion binding
metal ion binding
protein heterodimerization activity
hydrolase activity
primary active transporter
ion transport
potassium ion transport
cellular sodium ion homeostasis
response to xenobiotic stimulus
regulation of proton transport
establishment or maintenance of transmembrane electrochemical gradient
cellular potassium ion homeostasis
ion transmembrane transport
sodium ion export from cell
pH reduction
potassium ion transmembrane transport
hydrogen ion transmembrane transport
potassium ion import across plasma membrane
gastric acid secretion
metabolic process
ATP hydrolysis coupled proton transport
sodium ion transmembrane transport
response to drug
transport
sodium ion transport
proton transport
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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