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|Tested species reactivity||Fruit fly|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to a portion of Drosophila ATP5E (within residues 1-50).|
|Purification||Antigen affinity chromatography|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:500-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody does not react with human samples.
Suggested positive control: Drosophila melanogaster extracts.
The Drosophila gene for the ATP synthase epsilon subunit (ATP5E) is known as stunted or sun (Kidd et al. 2005). Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.