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Invitrogen
ATP5I Polyclonal Antibody
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FIGURE: 1 / 2
ATP5I Antibody (PA5-101439) in ICC/IF
Immunocytochemistry analysis of ATP5I in Hela cells. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25°C. Samples were incubated with ATP5I polyclonal antibody (Product # PA5-101439) and mouse anti-beta tubulin antibody for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) (Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) (Green) were used as the secondary antibodies. The nuclear counter stain is DAPI (blue).
Product Details
PA5-101439
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human ATP5I(Accession P56385), corresponding to amino acid residues E38-K69.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total ATP5I.
Target Information
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATP synthase e chain, mitochondrial; ATP synthase membrane subunit e; ATP synthase subunit e, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; ATPase subunit e; F1F0-ATP synthase, murine e subunit
Gene Aliases: ATP5I; ATP5K; ATP5ME
UniProt ID: (Human) P56385
Entrez Gene ID: (Human) 521
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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