|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 50-100 of human ATP5I|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
This antibody detects endogenous protein at a molecular weight of 8 kDa.
Purity is >95% by SDS-PAGE.
Mitochondrial ATP synthases (ATPases) transduce the energy contained in membrane electrochemical proton gradients into the energy required for synthesis of high-energy phosphate bonds. ATPases contain two linked complexes: F1, the hydrophilic catalytic core; and F0, the membrane-embedded protein channel. The two complexes are linked by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled, via a rotary mechanism of the central stalk subunits, with proton translocation across the membrane. ATP5I, also known as mitochondrial ATP synthase subunit E or ATP5K, is a 69 amino acid protein member of the ATPase E subunit family. Localized to the inner membrane of the mitochondria, ATP5I is a part of the F0 complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ATP synthase e chain, mitochondrial; ATP synthase subunit e; ATP synthase subunit e, mitochondrial; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit E; ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e; ATP synthase, H+ transporting, mitochondrial Fo complex, subunit E; ATP5I; ATP5K; ATPase subunit e; F1F0-ATP synthase, murine e subunit; mitochondrial
Gene Aliases: 2610008D24Rik; ATP5I; ATP5K; Lfm-1; Lfm1
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