Invitrogen

ATP6IP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (10) ATP6IP2 antibodies

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ATP6IP2 Antibody in Western Blot (WB) — Western blot was performed using Anti-ATP6IP2 Polyclonal Antibody (Product # PA1-32079) and ~37 kDa band corresponding to ATP6IP2 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), PC-3 (Lane 3), SiHa (Lane 4), Panc-1 (Lane 5) and HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ATP6IP2 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of ATP6V1A was performed in HeLa cells (fixed with PFA, permeabilized with 0.5% Triton) using a ATP6V1A Monoclonal Antibody (GT1561) (Product # MA5-31554) (Blue) at a dilution of 10 µg/mL. Black : Isotype control.

ATP6IP2 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with ATP6IP2 siRNA and a decrease in signal intensity was observed in western blot application using Anti-ATP6IP2 Polyclonal Antibody (Product # PA1-32079). {KD}

Product Details

PA1-32079

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunohistochemistry (IHC)	1:10-1:500	-
Flow Cytometry (Flow)	10 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Goat / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide with sequence SIIYRMTNQKIRMD, from C Terminus of the protein sequence according to NP_005756 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	TBS, pH 7.3, with 0.5% BSA
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2062203

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX25959-PEP)..

Target Information

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: (pro)renin receptor; ATP6M8-9; ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H(+)-transporting, lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 1; ATPase, H+ transporting, lysosomal interacting protein 2; Embryonic liver differentiation factor 10; ER-localized type I transmembrane adapter; ER-localized type I transmembrane adaptor; MGC99577; N14F; prorenin receptor; Renin receptor; Renin receptor C-terminal fragment; Renin receptor cytoplasmic fragment; Renin receptor extracellular fragment; Renin receptor N-terminal fragment; Renin/prorenin receptor; V-ATPase M8.9 subunit; Vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9

Gene Aliases: (P)RR; 5730403E06Rik; APT6M8-9; ATP6AP2; ATP6IP2; ATP6M8-9; CAPER; ELDF10; HT028; M8-9; MRXE; MRXSH; MSTP009; PRR; PSEC0072; RENR; XMRE; XPDS

UniProt ID: (Human) O75787, (Rat) Q6AXS4, (Mouse) Q9CYN9

Entrez Gene ID: (Human) 10159, (Rat) 302526, (Mouse) 70495

Molecular Function: transmembrane signal receptor