Invitrogen

ATP6IP2 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (8) ATP6IP2 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

ATP6IP2 Antibody in Western Blot (WB) — Western blot was performed using Anti-ATP6IP2 Polyclonal Antibody (Product # PA1-32079) and ~37 kDa band corresponding to ATP6IP2 was observed across the panel tested. Whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), T-47D (Lane 2), PC-3 (Lane 3), SiHa (Lane 4), Panc-1 (Lane 5) and HCT 116 (Lane 6) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 µg/mL) and detected by chemiluminescence with Rabbit anti-Goat IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27014, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

ATP6IP2 Antibody in Flow Cytometry (Flow) — Flow Cytometry analysis of ATP6V1A was performed in HeLa cells (fixed with PFA, permeabilized with 0.5% Triton) using a ATP6V1A Monoclonal Antibody (GT1561) (Product # MA5-31554) (Blue) at a dilution of 10 µg/mL. Black : Isotype control.

ATP6IP2 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. MCF-7 cells were transfected with ATP6IP2 siRNA and a decrease in signal intensity was observed in western blot application using Anti-ATP6IP2 Polyclonal Antibody (Product # PA1-32079). {KD}

Product Details

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

10 µg/mL

Immunohistochemistry (IHC)

1:10-1:500

Western Blot (WB)

1-2 µg/mL

Product Specifications

Species

Human, Mouse, Rat

Expression System

Goat / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Peptide with sequence SIIYRMTNQKIRMD, from C Terminus of the protein sequence according to NP_005756

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

TBS, pH 7.3, with 0.5% BSA

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2062203

Product Specific Information

Recommended positive controls: The peptide used to generate this antibody is available for purchase (GTX25959-PEP)..

Target Information

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

References (0)

Have you cited this product in a publication?

Let us know so we can reference it here.

Cite this antibody

Bioinformatics

Protein Aliases: (pro)renin receptor; ATP6M8-9; ATPase H(+)-transporting lysosomal accessory protein 2; ATPase H(+)-transporting lysosomal-interacting protein 2; ATPase, H(+)-transporting, lysosomal-interacting protein 2; ATPase, H+ transporting, lysosomal (vacuolar proton pump) membrane sector associated protein M8-9; ATPase, H+ transporting, lysosomal accessory protein 2; ATPase, H+ transporting, lysosomal interacting protein 1; ATPase, H+ transporting, lysosomal interacting protein 2; Embryonic liver differentiation factor 10; ER-localized type I transmembrane adaptor; MGC99577; N14F; prorenin receptor; Renin receptor; Renin receptor C-terminal fragment; Renin receptor cytoplasmic fragment; Renin receptor extracellular fragment; Renin receptor N-terminal fragment; Renin/prorenin receptor; V-ATPase M8.9 subunit; Vacuolar ATP synthase membrane sector-associated protein M8-9; vacuolar proton ATP synthase membrane sector associated protein M8-9

Gene Aliases: (P)RR; 5730403E06Rik; APT6M8-9; ATP6AP2; ATP6IP2; ATP6M8-9; CAPER; ELDF10; HT028; M8-9; MRXE; MRXSH; MSTP009; PRR; PSEC0072; RENR; XMRE; XPDS

UniProt ID: (Human) O75787, (Rat) Q6AXS4, (Mouse) Q9CYN9

Entrez Gene ID: (Human) 10159, (Rat) 302526, (Mouse) 70495

Function(s)

angiotensin maturation proteolysis biological_process rostrocaudal neural tube patterning positive regulation of Wnt signaling pathway positive regulation of transforming growth factor beta1 production regulation of MAPK cascade eye pigmentation head morphogenesis

Process(es)

molecular_function aspartic-type endopeptidase activity receptor activity enzyme binding