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ATP6V0A4 Antibody (PA5-29109) in ICC/IF
ATP6V0A4 Polyclonal Antibody detects ATP6V0A4 protein at cytoplasm by immunofluorescent analysis. Sample: A431 cells were fixed in ice-cold MeOH for 5 min. Green: ATP6V0A4 protein stained by ATP6V0A4 Polyclonal Antibody (Product # PA5-29109) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29109
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fragment corresponding to a region within amino acids 53 and 333 of Human ATP6V0A4
Conjugate
Form
Liquid
Concentration
0.65 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2546585
Product Specific Information
Recommended positive controls: 293T, A431, H1299, HeLa, Molt-4, Raji.
Predicted reactivity: Mouse (89%), Bovine (84%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing. [provided by RefSeq, Jul 2008]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 1B; ATPase, H+ transporting, lysosomal (vacuolar proton pump) non-catalytic accessory protein 2 (38kD); ATPase, H+ transporting, lysosomal V0 subunit a4; H(+)-transporting two-sector ATPase, noncatalytic accessory protein 1B; tcag7.346; V-ATPase 116 kDa; V-ATPase 116 kDa isoform a 4; V-type proton ATPase 116 kDa subunit a; V-type proton ATPase 116 kDa subunit a 4; vacuol; vacuolar proton pump 116 kDa accessory subunit; vacuolar proton pump, subunit 2; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 4; Vacuolar proton translocating ATPase 116 kDa subunit a kidney isoform
Gene Aliases: A4; ATP6N1B; ATP6N2; ATP6V0A4; RDRTA2; RTA1C; RTADR; STV1; VPH1; VPP2
UniProt ID: (Human) Q9HBG4
Entrez Gene ID: (Human) 50617
ossification
regulation of pH
vacuolar acidification
excretion
sensory perception of sound
insulin receptor signaling pathway
ATP synthesis coupled proton transport
ATP hydrolysis coupled proton transport
proton transport
transferrin transport
ion transmembrane transport
vacuolar proton-transporting V-type ATPase complex assembly
phagosome acidification
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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